DBA object, after running getPeakProfiles
Specifically, it uses the element MD, which should contain an
element called according to fieldname.
Peak.id
integer specifying the index of the peak to be drawn.
Sample.ids
sample ids (as in Cfp1$samples$SampleID)
NormMethod
specify which normalization method should be used,
currently only the 'DESeq' method [3] is implemented. Note, that unless
NormMethod=NULL, getNormFactors has to be called first.
plot.input
TRUE, if the input (control) should be included on
the plot
fieldname
name of list element in DBA$MD that is used for
plotting of peak. (e.g. PeakRawHists)
save2file
if TRUE plot is saved to pdf file
fn.pics
name of pdf file, to which the plot will be saved to.
Author(s)
Gabriele Schweikert
See Also
getPeakProfiles,
getNormFactors,
plotHistDists
Examples
# load DBA objects with peak profiles
data(Cfp1Profiles)
plotPeak(Cfp1Profiles, Peak.id=20, NormMethod=NULL)
# plot normalized profiles of WT.AB2 and Resc.AB2 samples, don't plot
# the input:
Cfp1Norm <- getNormFactors(Cfp1Profiles)
Sample.ids <- c("WT.AB2", "Resc.AB2")
plotPeak(Cfp1Norm, Peak.id=20, Sample.ids=Sample.ids,
NormMethod='DESeq', plot.input = FALSE)
Results
R version 3.3.1 (2016-06-21) -- "Bug in Your Hair"
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Type 'demo()' for some demos, 'help()' for on-line help, or
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> library(MMDiff)
Loading required package: GenomicRanges
Loading required package: BiocGenerics
Loading required package: parallel
Attaching package: 'BiocGenerics'
The following objects are masked from 'package:parallel':
clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
clusterExport, clusterMap, parApply, parCapply, parLapply,
parLapplyLB, parRapply, parSapply, parSapplyLB
The following objects are masked from 'package:stats':
IQR, mad, xtabs
The following objects are masked from 'package:base':
Filter, Find, Map, Position, Reduce, anyDuplicated, append,
as.data.frame, cbind, colnames, do.call, duplicated, eval, evalq,
get, grep, grepl, intersect, is.unsorted, lapply, lengths, mapply,
match, mget, order, paste, pmax, pmax.int, pmin, pmin.int, rank,
rbind, rownames, sapply, setdiff, sort, table, tapply, union,
unique, unsplit
Loading required package: S4Vectors
Loading required package: stats4
Attaching package: 'S4Vectors'
The following objects are masked from 'package:base':
colMeans, colSums, expand.grid, rowMeans, rowSums
Loading required package: IRanges
Loading required package: GenomeInfoDb
Loading required package: DiffBind
Loading required package: SummarizedExperiment
Loading required package: Biobase
Welcome to Bioconductor
Vignettes contain introductory material; view with
'browseVignettes()'. To cite Bioconductor, see
'citation("Biobase")', and for packages 'citation("pkgname")'.
Loading required package: GMD
Loading required package: Rsamtools
Loading required package: Biostrings
Loading required package: XVector
Warning message:
Package 'MMDiff' is deprecated and will be removed from Bioconductor
version 3.4
> png(filename="/home/ddbj/snapshot/RGM3/R_BC/result/MMDiff/plotPeak.Rd_%03d_medium.png", width=480, height=480)
> ### Name: plotPeak
> ### Title: plot read enrichment profiles at a specific peak for all
> ### specified samples.
> ### Aliases: plotPeak
>
> ### ** Examples
>
>
> # load DBA objects with peak profiles
> data(Cfp1Profiles)
> plotPeak(Cfp1Profiles, Peak.id=20, NormMethod=NULL)
No normalization factors applied
>
> # plot normalized profiles of WT.AB2 and Resc.AB2 samples, don't plot
> # the input:
>
> Cfp1Norm <- getNormFactors(Cfp1Profiles)
Computing Scaling factor according to DESeq normalization method
Using all Samples: nSamples = 3
Samples:
[1] "WT.AB2" "Null.AB2" "Resc.AB2"
Using unfiltered Peaks
nPeaks = 1000 (of 1000)
appending NormTotalCounts
Determined Factors:
$`WT.AB2,Null.AB2,Resc.AB2`
WT_2 Null_2 Resc_2
0.9439729 0.8899213 1.1986945
> Sample.ids <- c("WT.AB2", "Resc.AB2")
> plotPeak(Cfp1Norm, Peak.id=20, Sample.ids=Sample.ids,
+ NormMethod='DESeq', plot.input = FALSE)
>
>
>
>
>
>
> dev.off()
null device
1
>