Last data update: 2014.03.03

R: plot read enrichment profiles at a specific peak for all...
plotPeakR Documentation

plot read enrichment profiles at a specific peak for all specified samples.

Description

plot read enrichment profiles at a specific peak for all specified samples.

Usage

plotPeak(DBA, Peak.id, Sample.ids = NULL, NormMethod = 'DESeq',
        plot.input = TRUE, fieldname = "PeakRawHists",
        save2file = FALSE, fn.pics)

Arguments

DBA

DBA object, after running getPeakProfiles Specifically, it uses the element MD, which should contain an element called according to fieldname.

Peak.id

integer specifying the index of the peak to be drawn.

Sample.ids

sample ids (as in Cfp1$samples$SampleID)

NormMethod

specify which normalization method should be used, currently only the 'DESeq' method [3] is implemented. Note, that unless NormMethod=NULL, getNormFactors has to be called first.

plot.input

TRUE, if the input (control) should be included on the plot

fieldname

name of list element in DBA$MD that is used for plotting of peak. (e.g. PeakRawHists)

save2file

if TRUE plot is saved to pdf file

fn.pics

name of pdf file, to which the plot will be saved to.

Author(s)

Gabriele Schweikert

See Also

getPeakProfiles, getNormFactors, plotHistDists

Examples


# load DBA objects with peak profiles 
data(Cfp1Profiles)
plotPeak(Cfp1Profiles, Peak.id=20, NormMethod=NULL)

# plot normalized profiles of WT.AB2 and Resc.AB2 samples,  don't plot
# the input:

Cfp1Norm <- getNormFactors(Cfp1Profiles)
Sample.ids <- c("WT.AB2", "Resc.AB2")
plotPeak(Cfp1Norm, Peak.id=20, Sample.ids=Sample.ids,
      NormMethod='DESeq', plot.input = FALSE)

Results


R version 3.3.1 (2016-06-21) -- "Bug in Your Hair"
Copyright (C) 2016 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu (64-bit)

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Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

> library(MMDiff)
Loading required package: GenomicRanges
Loading required package: BiocGenerics
Loading required package: parallel

Attaching package: 'BiocGenerics'

The following objects are masked from 'package:parallel':

    clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
    clusterExport, clusterMap, parApply, parCapply, parLapply,
    parLapplyLB, parRapply, parSapply, parSapplyLB

The following objects are masked from 'package:stats':

    IQR, mad, xtabs

The following objects are masked from 'package:base':

    Filter, Find, Map, Position, Reduce, anyDuplicated, append,
    as.data.frame, cbind, colnames, do.call, duplicated, eval, evalq,
    get, grep, grepl, intersect, is.unsorted, lapply, lengths, mapply,
    match, mget, order, paste, pmax, pmax.int, pmin, pmin.int, rank,
    rbind, rownames, sapply, setdiff, sort, table, tapply, union,
    unique, unsplit

Loading required package: S4Vectors
Loading required package: stats4

Attaching package: 'S4Vectors'

The following objects are masked from 'package:base':

    colMeans, colSums, expand.grid, rowMeans, rowSums

Loading required package: IRanges
Loading required package: GenomeInfoDb
Loading required package: DiffBind
Loading required package: SummarizedExperiment
Loading required package: Biobase
Welcome to Bioconductor

    Vignettes contain introductory material; view with
    'browseVignettes()'. To cite Bioconductor, see
    'citation("Biobase")', and for packages 'citation("pkgname")'.


Loading required package: GMD
Loading required package: Rsamtools
Loading required package: Biostrings
Loading required package: XVector
Warning message:
Package 'MMDiff' is deprecated and will be removed from Bioconductor
  version 3.4 
> png(filename="/home/ddbj/snapshot/RGM3/R_BC/result/MMDiff/plotPeak.Rd_%03d_medium.png", width=480, height=480)
> ### Name: plotPeak
> ### Title: plot read enrichment profiles at a specific peak for all
> ###   specified samples.
> ### Aliases: plotPeak
> 
> ### ** Examples
> 
> 
> # load DBA objects with peak profiles 
> data(Cfp1Profiles)
> plotPeak(Cfp1Profiles, Peak.id=20, NormMethod=NULL)
No normalization factors applied
> 
> # plot normalized profiles of WT.AB2 and Resc.AB2 samples,  don't plot
> # the input:
> 
> Cfp1Norm <- getNormFactors(Cfp1Profiles)
Computing Scaling factor according to DESeq normalization method
Using all Samples: nSamples = 3
Samples:
[1] "WT.AB2"   "Null.AB2" "Resc.AB2"
Using unfiltered Peaks
nPeaks = 1000 (of 1000)
appending NormTotalCounts
Determined Factors:
$`WT.AB2,Null.AB2,Resc.AB2`
     WT_2    Null_2    Resc_2 
0.9439729 0.8899213 1.1986945 

> Sample.ids <- c("WT.AB2", "Resc.AB2")
> plotPeak(Cfp1Norm, Peak.id=20, Sample.ids=Sample.ids,
+       NormMethod='DESeq', plot.input = FALSE)
> 
> 
> 
> 
> 
> 
> dev.off()
null device 
          1 
>