A Helicase Dependent Amplification (HDA) of HPRT1 (Homo sapiens hypoxanthine phosphoribosyltransferase 1) was performed at different temperatures in the VideoScan Platform 2.0 (similar to Roediger et al. (2013)). The HDA was performed at 55, 60 and 65 degrees Celsius. The optimal temperature for a HDA is circa 65 degrees Celsius. Lower temperatures will affect the slope and plateau of the HDA amplification curve.
● Data Source:
CranContrib
● Keywords: HDA, HPRT1, VideoScan, datasets
● Alias: C17
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Data set of an amplification reaction using the Bio-Rad CFX96 thermo cycler. The samples of Vimentin were amplified in the CFX96 as replicates according to Roediger et al. (2013). The quantification was performed during the annealing step (60 degrees Celsius).
● Data Source:
CranContrib
● Keywords: Annealing, CFX96, datasets
● Alias: VIMCFX96_60
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A Helicase Dependent Amplification (HDA) of HPRT1 (Homo sapiens hypoxanthine phosphoribosyltransferase 1) was performed at three different input DNA quantities using the Bio-Rad iQ5 thermo cycler. The HDA was performed at 65 degrees Celsius. The optimal temperature for a HDA is circa 65 degrees Celsius. Lower temperatures will affect the slope and plateau of the HDA amplification curve.
● Data Source:
CranContrib
● Keywords: datasets
● Alias: C67
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Data set of an amplification reaction using the Bio-Rad iQ5 thermo cycler. The samples of Vimentin were amplified in the iQ5 as replicates according to Roediger et al. (2013). The quantification was performed during the annealing step (59.5 degrees Celsius).
● Data Source:
CranContrib
● Keywords: Annealing, datasets, iQ5
● Alias: VIMiQ5_595
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Quantitative PCR (qPCR) with a hydrolysis probe (Cy5/BHQ2) and DNA binding dye (EvaGreen) (Mao et al. 2007) was performed in the Roche Light Cycler 1.5 thermo cycler. The cycle-dependent increase of the fluorescence was quantified at the annealing step.
● Data Source:
CranContrib
● Keywords: MLC-2v, TaqMan, datasets, qPCR
● Alias: C127EGHP
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Highly replicate number amplification curves with an approximate amplification efficiency of 100 percent at cycle number 18. The data were derived from a simulation such as the AmpSim function.
● Data Source:
CranContrib
● Keywords: datasets
● Alias: Eff1000
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A melting curve for adk was performed.
● Data Source:
CranContrib
● Keywords: EvaGreen, adk, amplification, datasets
● Alias: C317.melt.lr
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A melting curve for adk was performed.
● Data Source:
CranContrib
● Keywords: EvaGreen, adk, amplification, datasets
● Alias: C317.melt.hr
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Real-time amplification plot of an Recombinase Polymerase Amplification (RPA) by Lutz et al.(2009) in a centrifugal microfluidic foil cartridge.
● Data Source:
CranContrib
● Keywords: RPA, datasets, isothermal
● Alias: RPA
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Highly replicate number amplification curves with an approximate amplification efficiency of 62.5 percent at cycle number 18. The data were derived from a simulation such as the AmpSim function.
● Data Source:
CranContrib
● Keywords: datasets
● Alias: Eff625
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