The p-values provided here result from the following procedure: 1) simulation of two RNA-seq experiments with four replicates in each condition via the sim.function, 2) analysis of differentially expressed tags using the DESeq package.
Gamma regression parameters describing the mean-dispersion relationship for each of the two real datasets considered in the associated paper, as estimated using the DESeq package version 1.8.3 (Anders and Huber, 2010).