A helper to re-estimate the spot location or find the capture site location (BF image modelling).
● Data Source:
BioConductor
● Keywords: internal
● Alias: getCsFAIL
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It retrieves the run effect and background corrected signals.
● Data Source:
BioConductor
● Keywords:
● Alias: getFluo
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It produces the background corrected data when run correction is not needed. It can be used for data coming from a single run instead of Fluo_adjustment(). Alternatively, this function can be used to visualize the fluorescence densities of a single batch before deciding the form of the normalization model.
● Data Source:
BioConductor
● Keywords:
● Alias: getFluo_byRun
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Identifies one or multiple spot(s) in the image data matrix.
● Data Source:
BioConductor
● Keywords: internal
● Alias: getSpot
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It generates a coded message of the estimation type that is being performed.
● Data Source:
BioConductor
● Keywords: internal
● Alias: giveWarning
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It reconstructs the BF image characteristic lines.
● Data Source:
BioConductor
● Keywords: internal
● Alias: grouplines
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It performs the grubbs test for outliers.
● Data Source:
BioConductor
● Keywords: internal
● Alias: grubbs
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A helper to identify the vertical BF image characteristic lines.
● Data Source:
BioConductor
● Keywords: internal
● Alias: highlight.cols
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It back-transforms the transformed adjusted cell fluorescence signals of a matrix.
● Data Source:
BioConductor
● Keywords: internal
● Alias: invTransform
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The original function to perform isotone regression (Motakis et al 2006).
● Data Source:
BioConductor
● Keywords: internal
● Alias: isotone
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