Calibrate.sigmoid.curve is a utility function used to fit the parameters for the sigmoidparameters option within NAPPA controlling the degree of shrinkage applied to the housekeeping normalisation. This may be useful if applying NAPPA to a new sample type. It is recommended that this is only performed once a sizeable data corpis has been collected over multiple seperate experiments.
Enables the processing and normalisation of the mRNA data output from the Nanostring nCounter software. Performs an adjustment based on the observed field of view for each lane. Performs a background correction using the truncated Poisson distribution adjustment. Performs a positive control normalisation using the E2 value. Performs a housekeeper normalisation by estimating the slope multiplier from sigmoidal curve fit.
Performs postive control, negative control and housekeeping normalisation of Nanostring mRNA data. Enables multiple cartridges from the same codeset to be normalised as a single dataset.
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