This function estimates the variance stabilizing cofactors, one for each channel for the entire dataset. When a fluorescence channel z is transformed by asinh transformation with the optimum cofactor for z, the within-population variances of populations from all samples in the channel z are approximately stabilized.
This function transforms a flowSet by asinh transformation with the cofactors passed on to the function. The optimum cofactors that stabilize within-population variances in different fluorescence channels are estimated beforehand and passed to this function for data transformation.