R: Select one readout from the twice read data and store...
selectPlate
R Documentation
Select one readout from the twice read data and store selected values.
In those cases where an experiment is read twice, only one readout from each pair is selected.
The selection is based on presence of minimum number of "selection key" or read with
highest mean is picked in case of tie.
Description
Select one readout from the twice read data and store selected values.
In those cases where an experiment is read twice, only one readout from each pair is selected.
The selection is based on presence of minimum number of "selection key" or read with
highest mean is picked in case of tie.
A vector containing all barcodes of data. In this case it is the output of function "extractKey".
numberofrowsperplate
This argument is not needed when you call function "readFluostarPlates".
The number of rows depends upon the geometry of the plates. These are 16 in case of 384 well plates.
selectionkey
key Value, that is used to select one of double read outs.
doubleplateexperiment
This parameter value can either be TRUE or FALSE. It is TRUE when an experiment is read twice.
Value
A hash table of selected readouts. Note: Hash tables are used to store the FMCA data because it consists of a key
(barcode) and a corresponding data. If COMBIA is used for large amount of FMCA data then the data in the hash table will
be more transparent, logical and easy to access than matrices.
R version 3.3.1 (2016-06-21) -- "Bug in Your Hair"
Copyright (C) 2016 The R Foundation for Statistical Computing
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> library(COMBIA)
Loading required package: hash
hash-2.2.6 provided by Decision Patterns
Loading required package: gdata
gdata: read.xls support for 'XLS' (Excel 97-2004) files ENABLED.
gdata: read.xls support for 'XLSX' (Excel 2007+) files ENABLED.
Attaching package: 'gdata'
The following object is masked from 'package:stats':
nobs
The following object is masked from 'package:utils':
object.size
The following object is masked from 'package:base':
startsWith
Loading required package: lattice
Loading required package: latticeExtra
Loading required package: RColorBrewer
Loading required package: oro.nifti
oro.nifti 0.5.2
> png(filename="/home/ddbj/snapshot/RGM3/R_CC/result/COMBIA/selectPlate.Rd_%03d_medium.png", width=480, height=480)
> ### Name: selectPlate
> ### Title: Select one readout from the twice read data and store selected
> ### values. In those cases where an experiment is read twice, only one
> ### readout from each pair is selected. The selection is based on
> ### presence of minimum number of "selection key" or read with highest
> ### mean is picked in case of tie.
> ### Aliases: selectPlate
>
> ### ** Examples
>
> f <- system.file("/raw/optima.log", package="COMBIA")
> fileDF <- readFile(filename = f, separator = ",", noofrows_skip=0,
+ platetype="384")
> Generatedbarcode <- extractKey(keyposition = 2,
+ rawdata = fileDF, numberofrowsperplate = 17, doubleplateexperiment=TRUE)
> hashedplates <- selectPlate(rawdata=fileDF,
+ processedbarcode = Generatedbarcode, numberofrowsperplate=17,
+ selectionkey="65000", doubleplateexperiment = FALSE )
>
>
>
>
>
> dev.off()
null device
1
>