The CoverageBigWigFile class contains information on a BigWIG file and inherits fields from the BigWigFile class in the rtracklayer package.
Objects from the Class
Objects can be created by calls of the form CoverageBigWigFile(). This class represents an alignment in the BigWIG format for which each genomic position has a depth of coverage value associated.
The reads_mapped argument is optional but it is required if the normalization parameter is set in the cov.matrix and cov.interval functions
Slots
resource
A character string that details the path to the BigWIG file
reads_mapped
A number representing the number of reads aligned in the file. Default = 0
...
Additional arguments
Author(s)
Ernesto Lowy <ernestolowy@gmail.com>
References
WIG format specification: https://genome.ucsc.edu/FAQ/FAQformat.html
See Also
BigWigFile
CoverageBamFile
Examples
##get BigWIG test file
inputfile<-system.file("extdata","treat.bw",package="CoverageView")
#create a CoverageBigWigFile object
trm<-CoverageBigWigFile(inputfile)
#create a CoverageBigWigFile object including the information of the number of reads aligned
trm1<-CoverageBigWigFile(inputfile,reads_mapped=28654321)
##get BigWIG test file
inputfile<-system.file("extdata","treat.bw",package="CoverageView")
#create a CoverageBigWigFile object
trm<-CoverageBigWigFile(inputfile)
#create a CoverageBigWigFile object including the information of the number of reads aligned
trm1<-CoverageBigWigFile(inputfile,reads_mapped=28654321)
Results
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> library(CoverageView)
Loading required package: Rsamtools
Loading required package: GenomeInfoDb
Loading required package: stats4
Loading required package: BiocGenerics
Loading required package: parallel
Attaching package: 'BiocGenerics'
The following objects are masked from 'package:parallel':
clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
clusterExport, clusterMap, parApply, parCapply, parLapply,
parLapplyLB, parRapply, parSapply, parSapplyLB
The following objects are masked from 'package:stats':
IQR, mad, xtabs
The following objects are masked from 'package:base':
Filter, Find, Map, Position, Reduce, anyDuplicated, append,
as.data.frame, cbind, colnames, do.call, duplicated, eval, evalq,
get, grep, grepl, intersect, is.unsorted, lapply, lengths, mapply,
match, mget, order, paste, pmax, pmax.int, pmin, pmin.int, rank,
rbind, rownames, sapply, setdiff, sort, table, tapply, union,
unique, unsplit
Loading required package: S4Vectors
Attaching package: 'S4Vectors'
The following objects are masked from 'package:base':
colMeans, colSums, expand.grid, rowMeans, rowSums
Loading required package: IRanges
Loading required package: GenomicRanges
Loading required package: Biostrings
Loading required package: XVector
Loading required package: rtracklayer
Attaching package: 'CoverageView'
The following object is masked from 'package:rtracklayer':
export.wig
> png(filename="/home/ddbj/snapshot/RGM3/R_BC/result/CoverageView/CoverageBigWigFile-class.Rd_%03d_medium.png", width=480, height=480)
> ### Name: CoverageBigWigFile-class
> ### Title: Class '"CoverageBigWigFile"'
> ### Aliases: CoverageBigWigFile-class CoverageBigWigFile
> ### show,CoverageBigWigFile-method
>
> ### ** Examples
>
> ##get BigWIG test file
> inputfile<-system.file("extdata","treat.bw",package="CoverageView")
>
> #create a CoverageBigWigFile object
> trm<-CoverageBigWigFile(inputfile)
>
> #create a CoverageBigWigFile object including the information of the number of reads aligned
> trm1<-CoverageBigWigFile(inputfile,reads_mapped=28654321)
>
> ##get BigWIG test file
> inputfile<-system.file("extdata","treat.bw",package="CoverageView")
>
> #create a CoverageBigWigFile object
> trm<-CoverageBigWigFile(inputfile)
>
> #create a CoverageBigWigFile object including the information of the number of reads aligned
> trm1<-CoverageBigWigFile(inputfile,reads_mapped=28654321)
>
>
>
>
>
> dev.off()
null device
1
>