Last data update: 2014.03.03

R: read coverage from bedGraph files
coverageFromBedGraphR Documentation

read coverage from bedGraph files

Description

read coverage from bedGraph files and save as a list.

Usage

    coverageFromBedGraph(bedgraphs, tags, genome, 
                        hugeData=FALSE, BPPARAM=NULL, ...)

Arguments

bedgraphs

The file names of bedgraphs generated by bedtools. eg: bedtools genomecov -bg -split -ibam $bam -g mm10.size.txt > $bedgraph

tags

the names for each input bedgraphs

genome

an object of BSgenome

hugeData

is this dataset consume too much memory? if it is TRUE, the coverage will be saved into tempfiles.

BPPARAM

An optional BiocParallelParam instance determining the parallel back-end to be used during evaluation, or a list of BiocParallelParam instances, to be applied in sequence for nested calls to bplapply.

...

parameters can be passed into tempfile. This is useful when you submit huge dataset to cluster.

Value

return a list of coverage for each bedgraph files. For each item in the list, it is a list of coverage for each chromosome. And the chromosome must start from "chr".

Author(s)

Jianhong Ou

Examples

    if(interactive()){
        library(BSgenome.Mmusculus.UCSC.mm10)
        path <- file.path(find.package("InPAS"), "extdata")
        bedgraphs <- file.path(path, "Baf3.extract.bedgraph")
        data(utr3.mm10)
        tags <- "Baf3"
        genome <- BSgenome.Mmusculus.UCSC.mm10
        coverage <- 
            coverageFromBedGraph(bedgraphs, tags, genome, hugeData=FALSE)
    }

Results


R version 3.3.1 (2016-06-21) -- "Bug in Your Hair"
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Platform: x86_64-pc-linux-gnu (64-bit)

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Type 'demo()' for some demos, 'help()' for on-line help, or
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> library(InPAS)
Loading required package: Biobase
Loading required package: BiocGenerics
Loading required package: parallel

Attaching package: 'BiocGenerics'

The following objects are masked from 'package:parallel':

    clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
    clusterExport, clusterMap, parApply, parCapply, parLapply,
    parLapplyLB, parRapply, parSapply, parSapplyLB

The following objects are masked from 'package:stats':

    IQR, mad, xtabs

The following objects are masked from 'package:base':

    Filter, Find, Map, Position, Reduce, anyDuplicated, append,
    as.data.frame, cbind, colnames, do.call, duplicated, eval, evalq,
    get, grep, grepl, intersect, is.unsorted, lapply, lengths, mapply,
    match, mget, order, paste, pmax, pmax.int, pmin, pmin.int, rank,
    rbind, rownames, sapply, setdiff, sort, table, tapply, union,
    unique, unsplit

Welcome to Bioconductor

    Vignettes contain introductory material; view with
    'browseVignettes()'. To cite Bioconductor, see
    'citation("Biobase")', and for packages 'citation("pkgname")'.

Loading required package: GenomicRanges
Loading required package: S4Vectors
Loading required package: stats4

Attaching package: 'S4Vectors'

The following objects are masked from 'package:base':

    colMeans, colSums, expand.grid, rowMeans, rowSums

Loading required package: IRanges
Loading required package: GenomeInfoDb
Loading required package: GenomicFeatures
Loading required package: AnnotationDbi
> png(filename="/home/ddbj/snapshot/RGM3/R_BC/result/InPAS/coverageFromBedGraph.Rd_%03d_medium.png", width=480, height=480)
> ### Name: coverageFromBedGraph
> ### Title: read coverage from bedGraph files
> ### Aliases: coverageFromBedGraph
> ### Keywords: misc
> 
> ### ** Examples
> 
> #    if(interactive()){
>         library(BSgenome.Mmusculus.UCSC.mm10)
Loading required package: BSgenome
Loading required package: Biostrings
Loading required package: XVector
Loading required package: rtracklayer
>         path <- file.path(find.package("InPAS"), "extdata")
>         bedgraphs <- file.path(path, "Baf3.extract.bedgraph")
>         data(utr3.mm10)
>         tags <- "Baf3"
>         genome <- BSgenome.Mmusculus.UCSC.mm10
>         coverage <- 
+             coverageFromBedGraph(bedgraphs, tags, genome, hugeData=FALSE)
> #    }
> 
> 
> 
> 
> 
> dev.off()
null device 
          1 
>