The file names of bedgraphs generated by bedtools.
eg: bedtools genomecov -bg -split -ibam $bam -g mm10.size.txt >
$bedgraph
tags
the names for each input bedgraphs
genome
an object of BSgenome
hugeData
is this dataset consume too much memory?
if it is TRUE, the coverage will be saved into tempfiles.
BPPARAM
An optional BiocParallelParam
instance determining the parallel back-end to be used during
evaluation, or a list of BiocParallelParam instances, to be applied
in sequence for nested calls to bplapply.
...
parameters can be passed into tempfile.
This is useful when you submit huge dataset to cluster.
Value
return a list of coverage for each bedgraph files.
For each item in the list, it is a list of coverage for each chromosome.
And the chromosome must start from "chr".
R version 3.3.1 (2016-06-21) -- "Bug in Your Hair"
Copyright (C) 2016 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu (64-bit)
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Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.
Type 'demo()' for some demos, 'help()' for on-line help, or
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> library(InPAS)
Loading required package: Biobase
Loading required package: BiocGenerics
Loading required package: parallel
Attaching package: 'BiocGenerics'
The following objects are masked from 'package:parallel':
clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
clusterExport, clusterMap, parApply, parCapply, parLapply,
parLapplyLB, parRapply, parSapply, parSapplyLB
The following objects are masked from 'package:stats':
IQR, mad, xtabs
The following objects are masked from 'package:base':
Filter, Find, Map, Position, Reduce, anyDuplicated, append,
as.data.frame, cbind, colnames, do.call, duplicated, eval, evalq,
get, grep, grepl, intersect, is.unsorted, lapply, lengths, mapply,
match, mget, order, paste, pmax, pmax.int, pmin, pmin.int, rank,
rbind, rownames, sapply, setdiff, sort, table, tapply, union,
unique, unsplit
Welcome to Bioconductor
Vignettes contain introductory material; view with
'browseVignettes()'. To cite Bioconductor, see
'citation("Biobase")', and for packages 'citation("pkgname")'.
Loading required package: GenomicRanges
Loading required package: S4Vectors
Loading required package: stats4
Attaching package: 'S4Vectors'
The following objects are masked from 'package:base':
colMeans, colSums, expand.grid, rowMeans, rowSums
Loading required package: IRanges
Loading required package: GenomeInfoDb
Loading required package: GenomicFeatures
Loading required package: AnnotationDbi
> png(filename="/home/ddbj/snapshot/RGM3/R_BC/result/InPAS/coverageFromBedGraph.Rd_%03d_medium.png", width=480, height=480)
> ### Name: coverageFromBedGraph
> ### Title: read coverage from bedGraph files
> ### Aliases: coverageFromBedGraph
> ### Keywords: misc
>
> ### ** Examples
>
> # if(interactive()){
> library(BSgenome.Mmusculus.UCSC.mm10)
Loading required package: BSgenome
Loading required package: Biostrings
Loading required package: XVector
Loading required package: rtracklayer
> path <- file.path(find.package("InPAS"), "extdata")
> bedgraphs <- file.path(path, "Baf3.extract.bedgraph")
> data(utr3.mm10)
> tags <- "Baf3"
> genome <- BSgenome.Mmusculus.UCSC.mm10
> coverage <-
+ coverageFromBedGraph(bedgraphs, tags, genome, hugeData=FALSE)
> # }
>
>
>
>
>
> dev.off()
null device
1
>