Microbead Surface Nucleic Acid Melting Curve Analysis

Description

Nucleic acid Melting Curve Analysis is a powerful method to investigate the interaction of double stranded nucleic acids. The MBmca package provides data sets and lightweight utilities for nucleic acid melting curve analysis and presentation on microbead surfaces. However, the function of the package can also be used for the analysis of reactions in solution (e.g., qPCR). Methods include melting curve data pre-processing (smooth, normalize, rotate, background subtraction), data inspection (comparison of multiplex melting curves) with location parameters (mean, median), deviation parameters (standard of the melting peaks including the second derivative. The second derivative melting peaks is implemented as parameter to further characterize the melting behavior. Plot functions to illustrate data quality, smoothed curves and derivatives are available too.

Details

Author(s)

Stefan Roediger <stefan_roediger@gmx.de>

References

A Highly Versatile Microscope Imaging Technology Platform for the Multiplex Real-Time Detection of Biomolecules and Autoimmune Antibodies. S. Roediger, P. Schierack, A. Boehm, J. Nitschke, I. Berger, U. Froemmel, C. Schmidt, M. Ruhland, I. Schimke, D. Roggenbuck, W. Lehmann and C. Schroeder. Advances in Biochemical Bioengineering/Biotechnology. 133:35–74, 2013. http://www.ncbi.nlm.nih.gov/pubmed/22437246

Surface Melting Curve Analysis with R. S. Roediger, A. Boehm and I. Schimke. The R Journal. 5(2):37–52, 2013. http://journal.r-project.org

Nucleic acid detection based on the use of microbeads: a review. S. Roediger, C. Liebsch, C. Schmidt, W. Lehmann, U. Resch-Genger, U. Schedler, P. Schierack. Microchim Acta 2014:1–18. DOI: 10.1007/s00604-014-1243-4

Results