R: Surface melting curve data from direct hybridization...
MultiMelt
R Documentation
Surface melting curve data from direct hybridization experiment of short
oligonucleotides.
Description
A melting curve experiment with twelve microbead populations and the short
oligonucleotide capture probe and detection probe for human HRPT1
(hyperparathyroidism 1) and MLC-2v (myosin regulatory light chain 2,
ventricular/cardiac muscle isoform).
Format
A data frame with the melting curves of two different capture and
detection probe pairs for HRPT1 and MLC-2v. First column contains the
temperature (in degree Celsius, 1 degree Celsius per step) followed by
melting curves of HRPT1 on twelve microbead populations and melting curves
of MLC-2v on twelve microbead populations.
T
a
numeric vector for the temperature in degree Celsius
HPRT1.1
a numeric vector, as HPRT1.1 of detection/capture
probe HPRT1/HPRT1-cap on microbead population 1
HPRT1.2
a
numeric vector, as HPRT1.2 on microbead population 2
HPRT1.3
a numeric vector, as HPRT1.3 on microbead population
3
HPRT1.4
a numeric vector, as HPRT1.4 on microbead
population 4
HPRT1.5
a numeric vector, as HPRT1.5 on
microbead population 5
HPRT1.6
a numeric vector, as HPRT1.6
on microbead population 6
HPRT1.7
a numeric vector, as
HPRT1.7 on microbead population 7
HPRT1.8
a numeric vector,
as HPRT1.8 on microbead population 8
HPRT1.9
a numeric
vector, as HPRT1.9 on microbead population 9
HPRT1.10
a
numeric vector, as HPRT1.10 on microbead population 10
HPRT1.11
a numeric vector, as HPRT1.11 on microbead
population 11
HPRT1.12
a numeric vector, as HPRT1.12 on
microbead population 12
MLC2v1
a numeric vector, as MLC2v1
of detection/capture probe MLC-2v/MLC-2v-cap on microbead population 1
MLC2v2
a numeric vector, as MLC2v2 on microbead population 2
MLC2v3
a numeric vector, as MLC2v3 on microbead population 3
MLC2v4
a numeric vector, as MLC2v4 on microbead population 4
MLC2v5
a numeric vector, as MLC2v5 on microbead population 5
MLC2v6
a numeric vector, as MLC2v6 on microbead population 6
MLC2v7
a numeric vector, as MLC2v7 on microbead population 7
MLC2v8
a numeric vector, as MLC2v8 on microbead population 8
MLC2v9
a numeric vector, as MLC2v9 on microbead population 9
MLC2v10
a numeric vector, as MLC2v10 on microbead population
10
MLC2v11
a numeric vector, as MLC2v11 on microbead
population 11
MLC2v12
a numeric vector, as MLC2v12 on
microbead population 12
Details
The melting curve was conducted with short oligonucleotide probes on the
surface of microbeads using the VideoScan platform according to Roediger et
al. (2012). The dyes and quencher used were Atto 647N and BHQ2.
Source
Data were measured with the VideoScan platform:
A Highly Versatile Microscope Imaging Technology Platform for the Multiplex
Real-Time Detection of Biomolecules and Autoimmune Antibodies. S. Roediger,
P. Schierack, A. Boehm, J. Nitschke, I. Berger, U. Froemmel, C. Schmidt, M.
Ruhland, I. Schimke, D. Roggenbuck, W. Lehmann and C. Schroeder.
Advances in Biochemical Bioengineering/Biotechnology. 133:35–74,
2013. http://www.ncbi.nlm.nih.gov/pubmed/22437246
Surface Melting Curve Analysis with R. S. Roediger, A. Boehm and I.
Schimke. The R Journal. 5(2):37–52, 2013, 2013.
http://journal.r-project.org/
R version 3.3.1 (2016-06-21) -- "Bug in Your Hair"
Copyright (C) 2016 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu (64-bit)
R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.
R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.
Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.
> library(MBmca)
Loading required package: robustbase
Loading required package: chipPCR
> png(filename="/home/ddbj/snapshot/RGM3/R_CC/result/MBmca/MultiMelt.Rd_%03d_medium.png", width=480, height=480)
> ### Name: MultiMelt
> ### Title: Surface melting curve data from direct hybridization experiment
> ### of short oligonucleotides.
> ### Aliases: MultiMelt
> ### Keywords: datasets
>
> ### ** Examples
>
> data(MultiMelt)
>
>
>
>
>
> dev.off()
null device
1
>