R: Generate a subdirectory,which is composed of QC Reports for...
Meth27QC
R Documentation
Generate a subdirectory,which is composed of QC Reports for Illuminas Infinium HumanMethylation27 BeadChip methylation assay
Description
Meth27QC is a tool analyzing Illumina Infinium HumanMethylation27 BeadChip Data and generating QC reports. This package allows users quickly assess data quality of the Assay. Users can evaluate the data quality in the way that Illumina GenomeStudio/BeadStudio recommended based on the control probes. The package reads files exported from GenomeStudio/BeadStudio software, generating intensity and standard deviation plots grouped by the types of the control probes. Meth27 carries 40 control probes for staining, hybridization, target removal, extension, bisulfite conversion, specificity, negative and non-polymorphic controls. Details of those control probes can be found in the Infinium Assay for Methylation Protocol Guide from Illumina.
We also used the other non-control probes to plot intensity of detected genes, signal average for green and red. Outliers can be identified.
Usage
Meth27QC(Dir,controlfile,sampfile)
Arguments
Dir
directory path where the control profile and sample file are included
controlfile
control probe file name,exported from BeadStudio/GenomeStudio
sampfile
sample table file name,exported from BeadStudio/GenomeStudio