List of 900 probes in the predictor data

Description

List of 900 probes in the predictor (Training data for Random Forest model)

Usage

data(MethPed_900probes)

Format

Data frame of 900 probes.

Details

For the construction of the training probe pool (predictors), methylation data generated by the Illumina Infinium HumanMethylation 450 BeadChip arrays were downloaded from the Gene Expression Omnibus (GEO). Four hundred seventy-two cases were available, representing several brain tumor diagnoses (DIPG, glioblastoma, ETMR, medulloblastoma, ependymoma, pilocytic astrocytoma) and their further subgroups.

The data sets were merged and probes that did not appear in all data sets were filtered away. In addition, about 190,000 CpGs were removed due to SNPs, repeats and multiple mapping sites. The final data set contained 206,823 unique probes and nine tumor classes including the medulloblastoma subgroups. K<c3><a2><c2><80><c2><93>neighbor imputation was used for missing probe data.

After that, a large number of regression analyses were performed to select the 100 probes per tumor class that had the highest predictive power (AUC values). Based on the identified 900 methylation sites, the nine pediatric brain tumor types could be accurately classified using the multiclass classification algorithm MethPed.

Value

450k methylation array probe name

References

[1] Anna Danielsson, Szil<c3><83><c2><a1>rd Nemes, Magnus Tisell, Birgitta Lannering, Claes Nordborg, Magnus Sabel, and Helena Car<c3><83><c2><a9>n. "MethPed: A DNA Methylation Classifier Tool for the Identification of Pediatric Brain Tumor Subtypes". Clinical Epigenetics 2015, 7:62, 2015

See Also

See http://www.clinicalepigeneticsjournal.com/content/7/1/62 for more details.

Examples

#################### Loading and view sample data
data(MethPed_900probes)
head(MethPed_900probes)

Results

R version 3.3.1 (2016-06-21) -- "Bug in Your Hair"
Copyright (C) 2016 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu (64-bit)

R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.

R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

> library(MethPed)
Loading required package: Biobase
Loading required package: BiocGenerics
Loading required package: parallel

Attaching package: 'BiocGenerics'

The following objects are masked from 'package:parallel':

    clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
    clusterExport, clusterMap, parApply, parCapply, parLapply,
    parLapplyLB, parRapply, parSapply, parSapplyLB

The following objects are masked from 'package:stats':

    IQR, mad, xtabs

The following objects are masked from 'package:base':

    Filter, Find, Map, Position, Reduce, anyDuplicated, append,
    as.data.frame, cbind, colnames, do.call, duplicated, eval, evalq,
    get, grep, grepl, intersect, is.unsorted, lapply, lengths, mapply,
    match, mget, order, paste, pmax, pmax.int, pmin, pmin.int, rank,
    rbind, rownames, sapply, setdiff, sort, table, tapply, union,
    unique, unsplit

Welcome to Bioconductor

    Vignettes contain introductory material; view with
    'browseVignettes()'. To cite Bioconductor, see
    'citation("Biobase")', and for packages 'citation("pkgname")'.

> png(filename="/home/ddbj/snapshot/RGM3/R_BC/result/MethPed/MethPed_900probes.Rd_%03d_medium.png", width=480, height=480)
> ### Name: MethPed_900probes
> ### Title: List of 900 probes in the predictor data
> ### Aliases: MethPed_900probes
> ### Keywords: data
> 
> ### ** Examples
> 
> #################### Loading and view sample data
> data(MethPed_900probes)
> head(MethPed_900probes)
    TargetID
1 cg00001930
2 cg00013409
3 cg00015639
4 cg00036119
5 cg00061695
6 cg00085790
> 
> 
> 
> 
> 
> 
> dev.off()
null device 
          1 
>