R: Map range coordinates between transcripts and genome space
mapToTranscripts
R Documentation
Map range coordinates between transcripts and genome space
Description
Map range coordinates between features in the transcriptome and
genome (reference) space.
See ?mapToAlignments in the
GenomicAlignments package for mapping coordinates between
reads (local) and genome (reference) space using a CIGAR alignment.
GRanges-class object of positions to be mapped.
x must have names when mapping to the genome.
transcripts
The OrganismDb object that will be used to extract features
using the extractor.fun.
ignore.strand
When TRUE, strand is ignored in overlap operations.
extractor.fun
Function to extract genomic features from a TxDb object.
Valid extractor functions:
transcripts ## default
exons
cds
genes
promoters
disjointExons
microRNAs
tRNAs
transcriptsBy
exonsBy
cdsBy
intronsByTranscript
fiveUTRsByTranscript
threeUTRsByTranscript
...
Additional arguments passed to extractor.fun functions.
Details
mapToTranscripts
The genomic range in x is mapped to the local position in the
transcripts ranges. A successful mapping occurs when x
is completely within the transcripts range, equivalent to:
findOverlaps(..., type="within")
Transcriptome-based coordinates start counting at 1 at the beginning
of the transcripts range and return positions where x
was aligned. The seqlevels of the return object are taken from the
transcripts object and should be transcript names. In this
direction, mapping is attempted between all elements of x and
all elements of transcripts.
Value
An object the same class as x.
Parallel methods return an object the same shape as x. Ranges that
cannot be mapped (out of bounds or strand mismatch) are returned as
zero-width ranges starting at 0 with a seqname of "UNMAPPED".
Non-parallel methods return an object that varies in length similar to a
Hits object. The result only contains mapped records, strand mismatch
and out of bound ranges are not returned. xHits and
transcriptsHits metadata columns indicate the elements of x
and transcripts used in the mapping.
When present, names from x are propagated to the output. When
mapping to transcript coordinates, seqlevels of the output are the names
on the transcripts object; most often these will be transcript
names. When mapping to the genome, seqlevels of the output are the seqlevels
of transcripts which are usually chromosome names.
Author(s)
V. Obenchain, M. Lawrence and H. Pages; ported to work with
OrganismDbi by Marc Carlson
See Also
mapToTranscripts .
Examples
## ---------------------------------------------------------------------
## A. Basic Use
## ---------------------------------------------------------------------
library(Homo.sapiens)
x <- GRanges("chr5",
IRanges(c(173315331,174151575), width=400,
names=LETTERS[1:2]))
## Map to transcript coordinates:
mapToTranscripts(x, Homo.sapiens)
Results
R version 3.3.1 (2016-06-21) -- "Bug in Your Hair"
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> library(OrganismDbi)
Loading required package: BiocGenerics
Loading required package: parallel
Attaching package: 'BiocGenerics'
The following objects are masked from 'package:parallel':
clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
clusterExport, clusterMap, parApply, parCapply, parLapply,
parLapplyLB, parRapply, parSapply, parSapplyLB
The following objects are masked from 'package:stats':
IQR, mad, xtabs
The following objects are masked from 'package:base':
Filter, Find, Map, Position, Reduce, anyDuplicated, append,
as.data.frame, cbind, colnames, do.call, duplicated, eval, evalq,
get, grep, grepl, intersect, is.unsorted, lapply, lengths, mapply,
match, mget, order, paste, pmax, pmax.int, pmin, pmin.int, rank,
rbind, rownames, sapply, setdiff, sort, table, tapply, union,
unique, unsplit
Loading required package: AnnotationDbi
Loading required package: stats4
Loading required package: Biobase
Welcome to Bioconductor
Vignettes contain introductory material; view with
'browseVignettes()'. To cite Bioconductor, see
'citation("Biobase")', and for packages 'citation("pkgname")'.
Loading required package: IRanges
Loading required package: S4Vectors
Attaching package: 'S4Vectors'
The following objects are masked from 'package:base':
colMeans, colSums, expand.grid, rowMeans, rowSums
Loading required package: GenomicFeatures
Loading required package: GenomeInfoDb
Loading required package: GenomicRanges
> png(filename="/home/ddbj/snapshot/RGM3/R_BC/result/OrganismDbi/coordinate-mapping-method.Rd_%03d_medium.png", width=480, height=480)
> ### Name: mapToTranscripts
> ### Title: Map range coordinates between transcripts and genome space
> ### Aliases: mapToTranscripts,ANY,MultiDb-method
> ### Keywords: methods utilities
>
> ### ** Examples
>
>
> ## ---------------------------------------------------------------------
> ## A. Basic Use
> ## ---------------------------------------------------------------------
>
> library(Homo.sapiens)
Loading required package: GO.db
Loading required package: org.Hs.eg.db
Loading required package: TxDb.Hsapiens.UCSC.hg19.knownGene
> x <- GRanges("chr5",
+ IRanges(c(173315331,174151575), width=400,
+ names=LETTERS[1:2]))
>
> ## Map to transcript coordinates:
> mapToTranscripts(x, Homo.sapiens)
GRanges object with 5 ranges and 2 metadata columns:
seqnames ranges strand | xHits transcriptsHits
<Rle> <IRanges> <Rle> | <integer> <integer>
A 21822 [1, 400] * | 1 21822
A 21823 [1, 400] * | 1 21823
A 21824 [1, 400] * | 1 21824
A 21825 [1, 400] * | 1 21825
B 21832 [1, 400] * | 2 21832
-------
seqinfo: 5 sequences from an unspecified genome; no seqlengths
>
>
>
>
>
> dev.off()
null device
1
>