Reads one or multiple ProteomicsAnnotationHubData dcf files into a
matrix that can be processed with
PAHD. Commnent lines starting with # will be
removed. See ProteomicsAnnotationDataHub() for details.
Usage
readPahdFiles(file)
Arguments
file
A character with one of multiple file names.
Value
A matrix containing
Author(s)
Laurent Gatto
Examples
## example file for the PXD000001 data
f <- list.files(system.file("extdata", package = "ProteomicsAnnotationHubData"),
full.names = TRUE, pattern = "PXD000001.dcf")
readPahdFiles(f)
Results
R version 3.3.1 (2016-06-21) -- "Bug in Your Hair"
Copyright (C) 2016 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu (64-bit)
R is free software and comes with ABSOLUTELY NO WARRANTY.
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Type 'license()' or 'licence()' for distribution details.
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Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.
Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.
> library(ProteomicsAnnotationHubData)
Loading required package: AnnotationHub
Loading required package: BiocGenerics
Loading required package: parallel
Attaching package: 'BiocGenerics'
The following objects are masked from 'package:parallel':
clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
clusterExport, clusterMap, parApply, parCapply, parLapply,
parLapplyLB, parRapply, parSapply, parSapplyLB
The following objects are masked from 'package:stats':
IQR, mad, xtabs
The following objects are masked from 'package:base':
Filter, Find, Map, Position, Reduce, anyDuplicated, append,
as.data.frame, cbind, colnames, do.call, duplicated, eval, evalq,
get, grep, grepl, intersect, is.unsorted, lapply, lengths, mapply,
match, mget, order, paste, pmax, pmax.int, pmin, pmin.int, rank,
rbind, rownames, sapply, setdiff, sort, table, tapply, union,
unique, unsplit
Loading required package: AnnotationHubData
Loading required package: S4Vectors
Loading required package: stats4
Attaching package: 'S4Vectors'
The following objects are masked from 'package:base':
colMeans, colSums, expand.grid, rowMeans, rowSums
Loading required package: IRanges
Loading required package: GenomicRanges
Loading required package: GenomeInfoDb
Setting options('download.file.method.GEOquery'='auto')
Setting options('GEOquery.inmemory.gpl'=FALSE)
This is ProteomicsAnnotationHubData version 1.2.2
Read 'ProteomicsAnnotationHubData()' to get started.
> png(filename="/home/ddbj/snapshot/RGM3/R_BC/result/ProteomicsAnnotationHubData/readPahdFiles.Rd_%03d_medium.png", width=480, height=480)
> ### Name: readPahdFiles
> ### Title: Reads one or multiple PAHD template files
> ### Aliases: readPahdFiles
>
> ### ** Examples
>
> ## example file for the PXD000001 data
> f <- list.files(system.file("extdata", package = "ProteomicsAnnotationHubData"),
+ full.names = TRUE, pattern = "PXD000001.dcf")
> readPahdFiles(f)
Title
[1,] "PXD000001: Erwinia carotovora and spiked-in protein fasta file"
[2,] "PXD000001: Peptide-level quantitation data"
[3,] "PXD000001: MS-GF+ identiciation data"
[4,] "PXD000001: raw mass spectrometry data"
Description
[1,] "Four human TMT spliked-in proteins in an Erwinia carotovora background. Expected reporter ion ratios: Erwinia peptides: 1:1:1:1:1:1; Enolase spike (sp|P00924|ENO1_YEAST): 10:5:2.5:1:2.5:10; BSA spike (sp|P02769|ALBU_BOVIN): 1:2.5:5:10:5:1; PhosB spike (sp|P00489|PYGM_RABIT): 2:2:2:2:1:1; Cytochrome C spike (sp|P62894|CYC_BOVIN): 1:1:1:1:1:2."
[2,] "Four human TMT spliked-in proteins in an Erwinia carotovora background. Expected reporter ion ratios: Erwinia peptides: 1:1:1:1:1:1; Enolase spike (sp|P00924|ENO1_YEAST): 10:5:2.5:1:2.5:10; BSA spike (sp|P02769|ALBU_BOVIN): 1:2.5:5:10:5:1; PhosB spike (sp|P00489|PYGM_RABIT): 2:2:2:2:1:1; Cytochrome C spike (sp|P62894|CYC_BOVIN): 1:1:1:1:1:2."
[3,] "Four human TMT spliked-in proteins in an Erwinia carotovora background. Expected reporter ion ratios: Erwinia peptides: 1:1:1:1:1:1; Enolase spike (sp|P00924|ENO1_YEAST): 10:5:2.5:1:2.5:10; BSA spike (sp|P02769|ALBU_BOVIN): 1:2.5:5:10:5:1; PhosB spike (sp|P00489|PYGM_RABIT): 2:2:2:2:1:1; Cytochrome C spike (sp|P62894|CYC_BOVIN): 1:1:1:1:1:2."
[4,] "Four human TMT spliked-in proteins in an Erwinia carotovora background. Expected reporter ion ratios: Erwinia peptides: 1:1:1:1:1:1; Enolase spike (sp|P00924|ENO1_YEAST): 10:5:2.5:1:2.5:10; BSA spike (sp|P02769|ALBU_BOVIN): 1:2.5:5:10:5:1; PhosB spike (sp|P00489|PYGM_RABIT): 2:2:2:2:1:1; Cytochrome C spike (sp|P62894|CYC_BOVIN): 1:1:1:1:1:2."
SourceType Recipe
[1,] "FASTA" "ProteomicsAnnotationHubData:::PXD000001FastaToAAStringSet"
[2,] "mzTab" "ProteomicsAnnotationHubData:::PXD000001MzTabToMSnSet"
[3,] "mzid" NA
[4,] "mzML" NA
RDataPath
[1,] "pride/data/archive/2012/03/PXD000001/erwinia_carotovora.rda"
[2,] "pride/data/archive/2012/03/PXD000001/F063721.dat-MSnSet.rda"
[3,] "pride/data/archive/2012/03/PXD000001/TMT_Erwinia_1uLSike_Top10HCD_isol2_45stepped_60min_01-20141210.mzid"
[4,] "pride/data/archive/2012/03/PXD000001/TMT_Erwinia_1uLSike_Top10HCD_isol2_45stepped_60min_01-20141210.mzML"
Location_Prefix SourceUrl Species TaxonomyId
[1,] "S3" "PRIDE" "Erwinia carotovora" "554"
[2,] "S3" "PRIDE" "Erwinia carotovora" "554"
[3,] "S3" "S3" "Erwinia carotovora" "554"
[4,] "PRIDE" "PRIDE" "Erwinia carotovora" "554"
File
[1,] "erwinia_carotovora.fasta"
[2,] "F063721.dat-mztab.txt"
[3,] "TMT_Erwinia_1uLSike_Top10HCD_isol2_45stepped_60min_01-20141210.mzid"
[4,] "TMT_Erwinia_1uLSike_Top10HCD_isol2_45stepped_60min_01-20141210.mzML"
DataProvider Maintainer RDataClass DispatchClass
[1,] "PRIDE" "Laurent Gatto <lg390@cam.ac.uk>" "AAStringSet" "AAStringSet"
[2,] "PRIDE" "Laurent Gatto <lg390@cam.ac.uk>" "MSnSet" "MSnSet"
[3,] "PRIDE" "Laurent Gatto <lg390@cam.ac.uk>" "mzRident" "mzRident"
[4,] "PRIDE" "Laurent Gatto <lg390@cam.ac.uk>" "mzRpwiz" "mzRpwiz"
Tags
[1,] "Proteomics, TMT6, LTQ Orbitrap Velos, PMID:23692960"
[2,] "Proteomics, TMT6, LTQ Orbitrap Velos, PMID:23692960"
[3,] "Proteomics, TMT6, LTQ Orbitrap Velos, PMID:23692960"
[4,] "Proteomics, TMT6, LTQ Orbitrap Velos, PMID:23692960"
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> dev.off()
null device
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