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Last data update: 2014.03.03

R: Gets fluorescence data vectors from 'RDML' object

RDML.GetFData

R Documentation

Gets fluorescence data vectors from `RDML` object

Description

Gets fluorescence data vectors from RDML object for specified method of experiment.

Arguments

`request`	Output from AsTable method(RDML.AsTable)
`limits`	`vector` with two values (min and max values) that limits cycles or temperature that should be gotten. If this values are smaller or bigger than min or max values at dats - NA will be used.
`data.type`	Type of fluorescence data (i.e. 'adp' for qPCR or 'mdp' for melting)
`long.table`	Output table is ready for ggplot (See RDML.AsTable for example)

Value

matrix which contains selected fluorescence data and additional information fromm request if long.table = TRUE.

Author(s)

Konstantin A. Blagodatskikh <k.blag@yandex.ru>, Stefan Roediger <stefan.roediger@b-tu.de>, Michal Burdukiewicz <michalburdukiewicz@gmail.com>

Examples

## Not run: 
## internal dataset BioRad_qPCR_melt.rdml (in 'data' directory)
## generated by Bio-Rad CFX96. Contains qPCR and melting data.
## Import without splitting by targets/types and with
## custom name pattern.
PATH <- path.package("RDML")
filename <- paste(PATH, "/extdata/", "BioRad_qPCR_melt.rdml", sep ="")
cfx96 <- RDML$new(filename)
## Select melting fluorescence data with sample.type 'unkn'.
library(dplyr)
tab <- cfx96$AsTable()
fdata <- cfx96$GetFData(filter(tab, sample.type == "unkn")
                        data.type = "mdp")
## Show names for getted fdata
colnames(fdata)

## End(Not run)