the header of a dataset file generated with generateDatasetFile
dataset
an R data frame generated with generateDatasetFile
listOfArgs
a list containing:
- a character string specifying the column whose values will be used for normalization
- a flag specifying whether controls should be excluded for the computation of the median polish (1) or not (0)
Value
A list containing:
header
The new header (with an added entry about the normalization procedure in the comments)
dataset
The new dataset with normalized values. The old values are saved in an extra column of the dataset with the suffix ".old"
References
C. Brideau, B. Gunter, B. Pikounis, and A. Liaw. Improved statistical methods for hit selection in high-throughput screening. J Biomol Screen, 8:634-647, 2003
R version 3.3.1 (2016-06-21) -- "Bug in Your Hair"
Copyright (C) 2016 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu (64-bit)
R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.
R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.
Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.
> library(RNAither)
Loading required package: topGO
Loading required package: BiocGenerics
Loading required package: parallel
Attaching package: 'BiocGenerics'
The following objects are masked from 'package:parallel':
clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
clusterExport, clusterMap, parApply, parCapply, parLapply,
parLapplyLB, parRapply, parSapply, parSapplyLB
The following objects are masked from 'package:stats':
IQR, mad, xtabs
The following objects are masked from 'package:base':
Filter, Find, Map, Position, Reduce, anyDuplicated, append,
as.data.frame, cbind, colnames, do.call, duplicated, eval, evalq,
get, grep, grepl, intersect, is.unsorted, lapply, lengths, mapply,
match, mget, order, paste, pmax, pmax.int, pmin, pmin.int, rank,
rbind, rownames, sapply, setdiff, sort, table, tapply, union,
unique, unsplit
Loading required package: graph
Loading required package: Biobase
Welcome to Bioconductor
Vignettes contain introductory material; view with
'browseVignettes()'. To cite Bioconductor, see
'citation("Biobase")', and for packages 'citation("pkgname")'.
Loading required package: GO.db
Loading required package: AnnotationDbi
Loading required package: stats4
Loading required package: IRanges
Loading required package: S4Vectors
Attaching package: 'S4Vectors'
The following objects are masked from 'package:base':
colMeans, colSums, expand.grid, rowMeans, rowSums
Loading required package: SparseM
Attaching package: 'SparseM'
The following object is masked from 'package:base':
backsolve
groupGOTerms: GOBPTerm, GOMFTerm, GOCCTerm environments built.
Attaching package: 'topGO'
The following object is masked from 'package:IRanges':
members
Loading required package: RankProd
Loading required package: prada
Loading required package: RColorBrewer
Loading required package: grid
Attaching package: 'grid'
The following object is masked from 'package:topGO':
depth
Loading required package: rrcov
Loading required package: robustbase
Attaching package: 'robustbase'
The following object is masked from 'package:Biobase':
rowMedians
Scalable Robust Estimators with High Breakdown Point (version 1.3-11)
Attaching package: 'RNAither'
The following object is masked from 'package:IRanges':
trim
> png(filename="/home/ddbj/snapshot/RGM3/R_BC/result/RNAither/BScore.Rd_%03d_medium.png", width=480, height=480)
> ### Name: BScore
> ### Title: BScore normalization
> ### Aliases: BScore
> ### Keywords: manip
>
> ### ** Examples
>
> data(exampleHeader, package="RNAither")
> data(exampleDataset, package="RNAither")
> normres <- BScore(header, dataset, list("SigIntensity", 0))
> newheader <- normres[[1]]
> newdataset <- normres[[2]]
>
>
>
>
>
> dev.off()
null device
1
>