Last data update: 2014.03.03

R: Plots the PCA scatterplots produced by codonPCA function.
printPCAR Documentation

Plots the PCA scatterplots produced by codonPCA function.

Description

Plots the PCA scatterplots produced by codonPCA function.

Usage

printPCA(listPCAGraphs)

Arguments

listPCAGraphs

a list of 5 PCA ggplot scatterplots.

Value

a unique plot with the 5 PCA scatterplots.

Examples

#How to perform a PCA analysis based on codon coverage
data(codonDataCtrl)
codonData <- codonDataCtrl
codonUsage <- codonData[[1]]
codonCovMatrix <- codonData[[2]]

#keep only genes with a minimum number of reads
nbrReadsGene <- apply(codonCovMatrix, 1, sum)
ixExpGenes <- which(nbrReadsGene >= 50)
codonCovMatrix <- codonCovMatrix[ixExpGenes, ]

#get the PCA on the codon coverage
codonCovMatrixTransp <- t(codonCovMatrix)
rownames(codonCovMatrixTransp) <- colnames(codonCovMatrix)
colnames(codonCovMatrixTransp) <- rownames(codonCovMatrix)

listPCACodonCoverage <- codonPCA(codonCovMatrixTransp,"codonCoverage")
printPCA(listPCACodonCoverage[[2]])

Results


R version 3.3.1 (2016-06-21) -- "Bug in Your Hair"
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> library(RiboProfiling)
Loading required package: Biostrings
Loading required package: BiocGenerics
Loading required package: parallel

Attaching package: 'BiocGenerics'

The following objects are masked from 'package:parallel':

    clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
    clusterExport, clusterMap, parApply, parCapply, parLapply,
    parLapplyLB, parRapply, parSapply, parSapplyLB

The following objects are masked from 'package:stats':

    IQR, mad, xtabs

The following objects are masked from 'package:base':

    Filter, Find, Map, Position, Reduce, anyDuplicated, append,
    as.data.frame, cbind, colnames, do.call, duplicated, eval, evalq,
    get, grep, grepl, intersect, is.unsorted, lapply, lengths, mapply,
    match, mget, order, paste, pmax, pmax.int, pmin, pmin.int, rank,
    rbind, rownames, sapply, setdiff, sort, table, tapply, union,
    unique, unsplit

Loading required package: S4Vectors
Loading required package: stats4

Attaching package: 'S4Vectors'

The following objects are masked from 'package:base':

    colMeans, colSums, expand.grid, rowMeans, rowSums

Loading required package: IRanges
Loading required package: XVector
Warning messages:
1: replacing previous import 'BiocGenerics::Position' by 'ggplot2::Position' when loading 'RiboProfiling' 
2: replacing previous import 'ggplot2::Position' by 'BiocGenerics::Position' when loading 'ggbio' 
> png(filename="/home/ddbj/snapshot/RGM3/R_BC/result/RiboProfiling/printPCA.Rd_%03d_medium.png", width=480, height=480)
> ### Name: printPCA
> ### Title: Plots the PCA scatterplots produced by codonPCA function.
> ### Aliases: printPCA
> 
> ### ** Examples
> 
> #How to perform a PCA analysis based on codon coverage
> data(codonDataCtrl)
> codonData <- codonDataCtrl
> codonUsage <- codonData[[1]]
> codonCovMatrix <- codonData[[2]]
> 
> #keep only genes with a minimum number of reads
> nbrReadsGene <- apply(codonCovMatrix, 1, sum)
> ixExpGenes <- which(nbrReadsGene >= 50)
> codonCovMatrix <- codonCovMatrix[ixExpGenes, ]
> 
> #get the PCA on the codon coverage
> codonCovMatrixTransp <- t(codonCovMatrix)
> rownames(codonCovMatrixTransp) <- colnames(codonCovMatrix)
> colnames(codonCovMatrixTransp) <- rownames(codonCovMatrix)
> 
> listPCACodonCoverage <- codonPCA(codonCovMatrixTransp,"codonCoverage")
> printPCA(listPCACodonCoverage[[2]])
TableGrob (1 x 2) "arrange": 2 grobs
  z     cells    name            grob
1 1 (1-1,1-1) arrange gtable[arrange]
2 2 (1-1,2-2) arrange gtable[arrange]
> 
> 
> 
> 
> 
> dev.off()
null device 
          1 
>