Function to convert an object of class MAList into an object of
class ExpressionSet. Note that the otherwise optional
targets component is required in this case to generate the
phenoData of the new ExpressionSet.
Usage
asExprSet(from, idColumn="PROBE_ID")
Arguments
from
object of class MAList to convert into an
ExpressionSet
idColumn
string; indicating which column of the genes
data.frame of the MAList holds the identifier for reporters on the
microarray. This column, after calling
make.names on it, will make up the unique
featureNames of the resulting ExpressionSet.
Value
an object of class ExpressionSet
Note
There is a more general function for converting MALists to
ExpressionSets in the package convert. This function here is
solely intended for converting Ringo-generated MALists into
ExpressionSets.
R version 3.3.1 (2016-06-21) -- "Bug in Your Hair"
Copyright (C) 2016 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu (64-bit)
R is free software and comes with ABSOLUTELY NO WARRANTY.
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R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.
Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.
> library(Ringo)
Loading required package: Biobase
Loading required package: BiocGenerics
Loading required package: parallel
Attaching package: 'BiocGenerics'
The following objects are masked from 'package:parallel':
clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
clusterExport, clusterMap, parApply, parCapply, parLapply,
parLapplyLB, parRapply, parSapply, parSapplyLB
The following objects are masked from 'package:stats':
IQR, mad, xtabs
The following objects are masked from 'package:base':
Filter, Find, Map, Position, Reduce, anyDuplicated, append,
as.data.frame, cbind, colnames, do.call, duplicated, eval, evalq,
get, grep, grepl, intersect, is.unsorted, lapply, lengths, mapply,
match, mget, order, paste, pmax, pmax.int, pmin, pmin.int, rank,
rbind, rownames, sapply, setdiff, sort, table, tapply, union,
unique, unsplit
Welcome to Bioconductor
Vignettes contain introductory material; view with
'browseVignettes()'. To cite Bioconductor, see
'citation("Biobase")', and for packages 'citation("pkgname")'.
Loading required package: RColorBrewer
Loading required package: limma
Attaching package: 'limma'
The following object is masked from 'package:BiocGenerics':
plotMA
Loading required package: Matrix
Loading required package: grid
Loading required package: lattice
> png(filename="/home/ddbj/snapshot/RGM3/R_BC/result/Ringo/asExprSet.Rd_%03d_medium.png", width=480, height=480)
> ### Name: asExprSet
> ### Title: converts a Ringo MAList into an ExpressionSet
> ### Aliases: asExprSet asExpressionSet
> ### Keywords: manip
>
> ### ** Examples
>
> exDir <- system.file("exData",package="Ringo")
> exRG <- readNimblegen("example_targets.txt","spottypes.txt",path=exDir)
Reading targets file...
Reading raw intensities...
Read header information
Read /home/ddbj/local/lib64/R/library/Ringo/exData/MOD_20551_PMT1_pair.txt
Read /home/ddbj/local/lib64/R/library/Ringo/exData/MOD_20742_PMT1_pair.txt
Determining probe categories...
Matching patterns for: GENE_EXPR_OPTION PROBE_ID
Found 991 Probe
Found 0 Probe
Found 0 Probe
Found 0 Negative
Found 0 Empty
Found 0 H_Code
Found 0 V_Code
Found 0 Random
Setting attributes: values Color
> exMA <- preprocess(exRG, "none", returnMAList=TRUE)
Normalizing...
> exX <- asExprSet(exMA)
>
>
>
>
>
> dev.off()
null device
1
>