Last data update: 2014.03.03

R: analytic function ratioTimeDependent
 ratioTimeDependent R Documentation

analytic function ratioTimeDependent

Description

Steady-state analytic solution to one-step model for the ratio of mature to non-mature fluorophores.

Usage

```ratioTimeDependent(T1, T2, halfLife, t, E=0, f=1)
```

Arguments

 `T1` Maturation time of fluorescent protein 1 (FP1, fast maturing). `T2` Maturation time of fluorescent protein 2 (FP2, slow maturing) `halfLife` Protein half-life. `t` Time, which must be non-negative. `E` FRET value representing energy transfer from FP1 to FP2. `f` f=f2/f1, the ratio of prefactors relating the number of molecules to fluorescence intensity.

Value

A numeric specifying the model time-dependent solution for the given parameters.

Joseph D. Barry

Examples

```tSeq <- seq(0.1, 300, by=0.1)
plot(tSeq, ratioTimeDependent(T1=5, T2=100, halfLife=30, t=tSeq, E=0, f=1), type="l", lwd=2,
xlab="time (min)", ylab="ratio", cex.lab=1.4, col="black", ylim=c(0, 0.3))
points(tSeq, ratioTimeDependent(T1=5, T2=100, halfLife=30, t=tSeq, E=0.4, f=1), type="l", lwd=2,
col="red")
abline(h=ratioSteadyState(T1=5, T2=100, halfLife=30, E=0, f=1), lty=2, col="black")
abline(h=ratioSteadyState(T1=5, T2=100, halfLife=30, E=0.4, f=1), lty=2, col="red")
```

Results

```
R version 3.3.1 (2016-06-21) -- "Bug in Your Hair"
Copyright (C) 2016 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu (64-bit)

R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.

R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

> library(TimerQuant)
Loading required package: shiny
> png(filename="/home/ddbj/snapshot/RGM3/R_BC/result/TimerQuant/ratioTimeDependent.Rd_%03d_medium.png", width=480, height=480)
> ### Name: ratioTimeDependent
> ### Title: analytic function ratioTimeDependent
> ### Aliases: ratioTimeDependent
> ### Keywords: ratioTimeDependent
>
> ### ** Examples
>
> tSeq <- seq(0.1, 300, by=0.1)
> plot(tSeq, ratioTimeDependent(T1=5, T2=100, halfLife=30, t=tSeq, E=0, f=1), type="l", lwd=2,
+     xlab="time (min)", ylab="ratio", cex.lab=1.4, col="black", ylim=c(0, 0.3))
> points(tSeq, ratioTimeDependent(T1=5, T2=100, halfLife=30, t=tSeq, E=0.4, f=1), type="l", lwd=2,
+     col="red")
> abline(h=ratioSteadyState(T1=5, T2=100, halfLife=30, E=0, f=1), lty=2, col="black")
> abline(h=ratioSteadyState(T1=5, T2=100, halfLife=30, E=0.4, f=1), lty=2, col="red")
>
>
>
>
>
> dev.off()
null device
1
>

```