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Last data update: 2014.03.03

R: analytic function ratioTimeDependent

ratioTimeDependent

R Documentation

analytic function ratioTimeDependent

Description

Steady-state analytic solution to one-step model for the ratio of mature to non-mature fluorophores.

Usage

ratioTimeDependent(T1, T2, halfLife, t, E=0, f=1)

Arguments

`T1`	Maturation time of fluorescent protein 1 (FP1, fast maturing).
`T2`	Maturation time of fluorescent protein 2 (FP2, slow maturing)
`halfLife`	Protein half-life.
`t`	Time, which must be non-negative.
`E`	FRET value representing energy transfer from FP1 to FP2.
`f`	f=f2/f1, the ratio of prefactors relating the number of molecules to fluorescence intensity.

Value

A numeric specifying the model time-dependent solution for the given parameters.

Author(s)

Joseph D. Barry

Examples

tSeq <- seq(0.1, 300, by=0.1)
plot(tSeq, ratioTimeDependent(T1=5, T2=100, halfLife=30, t=tSeq, E=0, f=1), type="l", lwd=2, 
    xlab="time (min)", ylab="ratio", cex.lab=1.4, col="black", ylim=c(0, 0.3))
points(tSeq, ratioTimeDependent(T1=5, T2=100, halfLife=30, t=tSeq, E=0.4, f=1), type="l", lwd=2, 
    col="red")
abline(h=ratioSteadyState(T1=5, T2=100, halfLife=30, E=0, f=1), lty=2, col="black")
abline(h=ratioSteadyState(T1=5, T2=100, halfLife=30, E=0.4, f=1), lty=2, col="red")

Results


R version 3.3.1 (2016-06-21) -- "Bug in Your Hair"
Copyright (C) 2016 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu (64-bit)

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> library(TimerQuant)
Loading required package: shiny
> png(filename="/home/ddbj/snapshot/RGM3/R_BC/result/TimerQuant/ratioTimeDependent.Rd_%03d_medium.png", width=480, height=480)
> ### Name: ratioTimeDependent
> ### Title: analytic function ratioTimeDependent
> ### Aliases: ratioTimeDependent
> ### Keywords: ratioTimeDependent
> 
> ### ** Examples
> 
> tSeq <- seq(0.1, 300, by=0.1)
> plot(tSeq, ratioTimeDependent(T1=5, T2=100, halfLife=30, t=tSeq, E=0, f=1), type="l", lwd=2, 
+     xlab="time (min)", ylab="ratio", cex.lab=1.4, col="black", ylim=c(0, 0.3))
> points(tSeq, ratioTimeDependent(T1=5, T2=100, halfLife=30, t=tSeq, E=0.4, f=1), type="l", lwd=2, 
+     col="red")
> abline(h=ratioSteadyState(T1=5, T2=100, halfLife=30, E=0, f=1), lty=2, col="black")
> abline(h=ratioSteadyState(T1=5, T2=100, halfLife=30, E=0.4, f=1), lty=2, col="red")
> 
> 
> 
> 
> 
> dev.off()
null device 
          1 
>