R: 384-well plate assay format to a 96-well plate library format
getLibraryPlate
R Documentation
384-well plate assay format to a 96-well plate library format
Description
Given a cellHTS object with data from an assay conducted in 384-well plate format, resulting from the combination of four consecutive 96-well plates of a reagent library, this function gives the plate identifiers for the 96-well plates.
Usage
getLibraryPlate(x)
Arguments
x
a cellHTS object.
Details
The cellHTS object x contains data from a screening experiment where every set of four consecutive 96-well plates was combined into a 384-well plate. Therefore, the only available plate identifiers are for the assay plate format (384-well plates). The way the four 96-well plates are transferred to a 384-well plate during an experiment is as follows: the robot stars by transferring the samples from the first 96-well plate into the first quadrant of the 384-well plate, and so on.
Value
An S3 object of class cellHTS, which extends the argument x by the following element:
libPlate
a vector of length equal to the total number of wells of all the 384-well plates, containing a number that identifies the 96-well plate. It ranges from 1 to four times the total number of 384-well plates.
data(KcViabSmall)
x <- getLibraryPlate(KcViabSmall)
table(x$libPlate)
Results
R version 3.3.1 (2016-06-21) -- "Bug in Your Hair"
Copyright (C) 2016 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu (64-bit)
R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.
R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.
Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.
> library(cellHTS)
Loading required package: grid
Warning message:
Package 'cellHTS' is deprecated and will be removed from Bioconductor
version 3.4. Please consider using 'cellHTS2' which offers better
functionality for working with multiple screens and with
multi-channel screens.
> png(filename="/home/ddbj/snapshot/RGM3/R_BC/result/cellHTS/getLibraryPlate.Rd_%03d_medium.png", width=480, height=480)
> ### Name: getLibraryPlate
> ### Title: 384-well plate assay format to a 96-well plate library format
> ### Aliases: getLibraryPlate
> ### Keywords: manip
>
> ### ** Examples
>
> data(KcViabSmall)
> x <- getLibraryPlate(KcViabSmall)
> table(x$libPlate)
1 2 3 4 5 6 7 8 9 10 11 12
96 96 96 96 96 96 96 96 96 96 96 96
>
>
>
>
>
> dev.off()
null device
1
>