Last data update: 2014.03.03

R: Creates a promoter/enhancer regions track from a file of...
DNaseI_RoadMapR Documentation

Creates a promoter/enhancer regions track from a file of RoadMap

Description

Creates a track of promoter/enhancer regions from a file of RoadMap using the Gviz bioconductor package. A complete list of features and their associated colours can be found in the user guide.

Usage

DNaseI_RoadMap(gen="hg19", chr, start, end, bedFilePath, featureDisplay='promotor',showId=TRUE, type_stacking="dense")

Arguments

gen

the name of the genome. Default value=hg19

chr

The chromosome of interest

start

The starting position in the region of interest (the smallest value)

end

The end position in the region of interest (the largest value)

bedFilePath

The file path to the .BED file containing the data to be visualised

featureDisplay

A vector of features to be displayed, such as 1_TssA. Spelling and capitalisation of features must be identical to those in the user guide (in the 'State & Acronym' column). There are three possibilities. First, the visualisation of only one feature (e.g. featureDisplay <- "1_TssA"), only the name of the specific feature is required. Second, visualisation of a set of features, for this a vector of features is required (e.g. featureDisplay <- c("1_TssA","2_TssAFlnk")). Finally, visualison all features in the genomic region, achived by using the word "all" (e.g. featureDisplay <- "all"), "all" is set by default. You can find the complete list of features and their associated colours in the user guide.

showId

Allows to visualise the Id of DNAse group.

type_stacking

Object of class"character", the stacking type of overlapping items on the final plot.One in c(hide, dense, squish, pack,full). More information cf the option "stacking" in Gviz

Value

An AnnotationTrack object of Gviz

Author(s)

Tiphaine Martin

Tom Hardiman

References

http://bioconductor.org/packages/release/bioc/html/Gviz.html

Got to RoadMap Epigenome

Examples

library("Gviz")
chr <- "chr1"
start <- 707612
end <- 722151
gen="hg19"

extdata <- system.file("extdata", package="coMET",mustWork=TRUE)
bedFilePath <- file.path(extdata, "RoadMap/regions_prom_E063.bed")

if(interactive()){
  DNaseI_RoadMapSingle <- DNaseI_RoadMap(gen,chr,start, end, bedFilePath, featureDisplay='promotor'  )
  plotTracks(DNaseI_RoadMapSingle, from = start, to = end)
} else {
  data(DNaseI_RoadMapSingle)
  plotTracks(DNaseI_RoadMapSingle, from = start, to = end)
}

Results


R version 3.3.1 (2016-06-21) -- "Bug in Your Hair"
Copyright (C) 2016 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu (64-bit)

R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.

R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

> library(coMET)
Loading required package: grid
Loading required package: biomaRt
Loading required package: Gviz
Loading required package: S4Vectors
Loading required package: stats4
Loading required package: BiocGenerics
Loading required package: parallel

Attaching package: 'BiocGenerics'

The following objects are masked from 'package:parallel':

    clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
    clusterExport, clusterMap, parApply, parCapply, parLapply,
    parLapplyLB, parRapply, parSapply, parSapplyLB

The following objects are masked from 'package:stats':

    IQR, mad, xtabs

The following objects are masked from 'package:base':

    Filter, Find, Map, Position, Reduce, anyDuplicated, append,
    as.data.frame, cbind, colnames, do.call, duplicated, eval, evalq,
    get, grep, grepl, intersect, is.unsorted, lapply, lengths, mapply,
    match, mget, order, paste, pmax, pmax.int, pmin, pmin.int, rank,
    rbind, rownames, sapply, setdiff, sort, table, tapply, union,
    unique, unsplit


Attaching package: 'S4Vectors'

The following objects are masked from 'package:base':

    colMeans, colSums, expand.grid, rowMeans, rowSums

Loading required package: IRanges
Loading required package: GenomicRanges
Loading required package: GenomeInfoDb
Loading required package: psych

Attaching package: 'psych'

The following object is masked from 'package:IRanges':

    reflect

Warning message:
replacing previous import 'ggplot2::Position' by 'BiocGenerics::Position' when loading 'ggbio' 
> png(filename="/home/ddbj/snapshot/RGM3/R_BC/result/coMET/DNaseI_RoadMap.Rd_%03d_medium.png", width=480, height=480)
> ### Name: DNaseI_RoadMap
> ### Title: Creates a promoter/enhancer regions track from a file of RoadMap
> ### Aliases: DNaseI_RoadMap
> ### Keywords: hplot
> 
> ### ** Examples
> 
> library("Gviz")
> chr <- "chr1"
> start <- 707612
> end <- 722151
> gen="hg19"
> 
> extdata <- system.file("extdata", package="coMET",mustWork=TRUE)
> bedFilePath <- file.path(extdata, "RoadMap/regions_prom_E063.bed")
> 
> #if(interactive()){
>   DNaseI_RoadMapSingle <- DNaseI_RoadMap(gen,chr,start, end, bedFilePath, featureDisplay='promotor'  )
Error in file(file, "rt") : cannot open the connection
Calls: DNaseI_RoadMap -> read.table -> file
In addition: Warning message:
In file(file, "rt") :
  cannot open file '/home/ddbj/local/lib64/R/library/coMET/extdata/RoadMap/regions_prom_E063.bed': No such file or directory
Execution halted