Creates an M vs A plot (Avg intensity vs log ratio) for a given pair of conditions across all fpkms
Usage
## S4 method for signature 'CuffData'
MAplot(object,x,y,logMode=T,pseudocount=1,smooth=FALSE,useCount=FALSE)
Arguments
object
An object of class 'CuffData'.
x
Sample name from 'samples' table for comparison
y
Sample name from 'samples' table for comparison
logMode
A logical argument to log10-transform FPKM values prior to plotting.
pseudocount
Value to be added to FPKM for appropriate log transformation and clustering. (Avoids zero-based errors)
smooth
Logical argument whether or not to draw a smoothed line fit through data.
useCount
Logical argument whether or not to use mean counts instead of FPKM values.
Details
None
Value
Returns a ggplot MvsA plot object.
Note
None
Author(s)
Loyal A. Goff and Cole Trapnell
References
None.
Examples
a<-readCufflinks(system.file("extdata", package="cummeRbund")) #Create CuffSet object from sample data
genes<-a@genes #Create CuffData object for all 'genes'
d<-MAplot(genes,'hESC','Fibroblasts') #Create csDensity plot
d #Render plot
Results
R version 3.3.1 (2016-06-21) -- "Bug in Your Hair"
Copyright (C) 2016 The R Foundation for Statistical Computing
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> library(cummeRbund)
Loading required package: BiocGenerics
Loading required package: parallel
Attaching package: 'BiocGenerics'
The following objects are masked from 'package:parallel':
clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
clusterExport, clusterMap, parApply, parCapply, parLapply,
parLapplyLB, parRapply, parSapply, parSapplyLB
The following objects are masked from 'package:stats':
IQR, mad, xtabs
The following objects are masked from 'package:base':
Filter, Find, Map, Position, Reduce, anyDuplicated, append,
as.data.frame, cbind, colnames, do.call, duplicated, eval, evalq,
get, grep, grepl, intersect, is.unsorted, lapply, lengths, mapply,
match, mget, order, paste, pmax, pmax.int, pmin, pmin.int, rank,
rbind, rownames, sapply, setdiff, sort, table, tapply, union,
unique, unsplit
Loading required package: RSQLite
Loading required package: DBI
Loading required package: ggplot2
Loading required package: reshape2
Loading required package: fastcluster
Attaching package: 'fastcluster'
The following object is masked from 'package:stats':
hclust
Loading required package: rtracklayer
Loading required package: GenomicRanges
Loading required package: S4Vectors
Loading required package: stats4
Attaching package: 'S4Vectors'
The following objects are masked from 'package:base':
colMeans, colSums, expand.grid, rowMeans, rowSums
Loading required package: IRanges
Loading required package: GenomeInfoDb
Loading required package: Gviz
Loading required package: grid
Attaching package: 'cummeRbund'
The following object is masked from 'package:GenomicRanges':
promoters
The following object is masked from 'package:IRanges':
promoters
The following object is masked from 'package:BiocGenerics':
conditions
> png(filename="/home/ddbj/snapshot/RGM3/R_BC/result/cummeRbund/MAplot.Rd_%03d_medium.png", width=480, height=480)
> ### Name: MAplot
> ### Title: MAplot
> ### Aliases: MAplot MAplot,CuffData-method
> ### Keywords: heatmap
>
> ### ** Examples
>
> a<-readCufflinks(system.file("extdata", package="cummeRbund")) #Create CuffSet object from sample data
Creating database /home/ddbj/local/lib64/R/library/cummeRbund/extdata/cuffData.db
Reading Run Info File /home/ddbj/local/lib64/R/library/cummeRbund/extdata/run.info
Writing runInfo Table
Reading Read Group Info /home/ddbj/local/lib64/R/library/cummeRbund/extdata/read_groups.info
Writing replicates Table
Reading /home/ddbj/local/lib64/R/library/cummeRbund/extdata/genes.fpkm_tracking
Checking samples table...
Populating samples table...
Writing genes table
Reshaping geneData table
Recasting
Writing geneData table
Reading /home/ddbj/local/lib64/R/library/cummeRbund/extdata/gene_exp.diff
Writing geneExpDiffData table
Reading /home/ddbj/local/lib64/R/library/cummeRbund/extdata/promoters.diff
Writing promoterDiffData table
Reading /home/ddbj/local/lib64/R/library/cummeRbund/extdata/genes.count_tracking
Reshaping geneCount table
Recasting
Writing geneCount table
Reading read group info in /home/ddbj/local/lib64/R/library/cummeRbund/extdata/genes.read_group_tracking
Writing geneReplicateData table
Reading /home/ddbj/local/lib64/R/library/cummeRbund/extdata/isoforms.fpkm_tracking
Checking samples table...
OK!
Writing isoforms table
Reshaping isoformData table
Recasting
Writing isoformData table
Reading /home/ddbj/local/lib64/R/library/cummeRbund/extdata/isoform_exp.diff
Writing isoformExpDiffData table
Reading /home/ddbj/local/lib64/R/library/cummeRbund/extdata/isoforms.count_tracking
Reshaping isoformCount table
Recasting
Writing isoformCount table
Reading read group info in /home/ddbj/local/lib64/R/library/cummeRbund/extdata/isoforms.read_group_tracking
Writing isoformReplicateData table
Reading /home/ddbj/local/lib64/R/library/cummeRbund/extdata/tss_groups.fpkm_tracking
Checking samples table...
OK!
Writing TSS table
Reshaping TSSData table
Recasting
Writing TSSData table
Reading /home/ddbj/local/lib64/R/library/cummeRbund/extdata/tss_group_exp.diff
Writing TSSExpDiffData table
Reading /home/ddbj/local/lib64/R/library/cummeRbund/extdata/splicing.diff
Writing splicingDiffData table
Reading /home/ddbj/local/lib64/R/library/cummeRbund/extdata/tss_groups.count_tracking
Reshaping TSSCount table
Recasting
Writing TSSCount table
Reading read group info in /home/ddbj/local/lib64/R/library/cummeRbund/extdata/tss_groups.read_group_tracking
Writing TSSReplicateData table
Reading /home/ddbj/local/lib64/R/library/cummeRbund/extdata/cds.fpkm_tracking
Checking samples table...
OK!
Writing CDS table
Reshaping CDSData table
Recasting
Writing CDSData table
Reading /home/ddbj/local/lib64/R/library/cummeRbund/extdata/cds_exp.diff
Writing CDSExpDiffData table
Reading /home/ddbj/local/lib64/R/library/cummeRbund/extdata/cds.diff
Writing CDSDiffData table
Reading /home/ddbj/local/lib64/R/library/cummeRbund/extdata/cds.count_tracking
Reshaping CDSCount table
Recasting
Writing CDSCount table
Reading read group info in /home/ddbj/local/lib64/R/library/cummeRbund/extdata/cds.read_group_tracking
Writing CDSReplicateData table
Indexing Tables...
Warning messages:
1: attributes are not identical across measure variables; they will be dropped
2: attributes are not identical across measure variables; they will be dropped
3: attributes are not identical across measure variables; they will be dropped
4: attributes are not identical across measure variables; they will be dropped
5: attributes are not identical across measure variables; they will be dropped
6: attributes are not identical across measure variables; they will be dropped
7: attributes are not identical across measure variables; they will be dropped
8: attributes are not identical across measure variables; they will be dropped
> genes<-a@genes #Create CuffData object for all 'genes'
> d<-MAplot(genes,'hESC','Fibroblasts') #Create csDensity plot
> d #Render plot
Warning message:
Removed 54 rows containing missing values (geom_point).
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> dev.off()
null device
1
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