Last data update: 2014.03.03

R: Density plot of CuffData
csDensityR Documentation

Density plot of CuffData

Description

Creates a smoothed density plot, by sample, for log10 FPKM values from a cuffdiff run.

Usage

## S4 method for signature 'CuffData'
csDensity(object, logMode=TRUE, pseudocount=0, labels, features=FALSE, replicates=FALSE,...)
## S4 method for signature 'CuffFeatureSet'
csDensity(object, logMode=TRUE, pseudocount=0, labels, features=FALSE, replicates=FALSE,...)

Arguments

object

An object of class CuffData.

logMode

A logical value of whether or not to log10-transform FPKM values. By default this is TRUE.

pseudocount

Pseudocount value added to FPKM to avoid errors in log-transformation of true zero values.

labels

A list of tracking_id values or gene_short_name values used for 'callout' points on the density plot for reference. (Not implemented yet).

features

Will include all fields from 'features' slot in returned ggplot object. Useful for further manipulations of plot object using feature-level attributes (e.g. gene_type, class_code, etc)

replicates

A logical value whether or not to plot individual replicates or aggregate condition values.

...

Additional arguments

Details

Creates a density plot, by sample, for log10-transformed FPKM values from a cuffdiff run.

Value

A ggplot2 plot object

Note

None

Author(s)

Loyal A. Goff

References

None

Examples

	a<-readCufflinks(system.file("extdata", package="cummeRbund")) #Create CuffSet object from sample data
	genes<-a@genes #Create CuffData object for all 'genes'
	d<-csDensity(genes) #Create csDensity plot
	d #Render plot

Results


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> library(cummeRbund)
Loading required package: BiocGenerics
Loading required package: parallel

Attaching package: 'BiocGenerics'

The following objects are masked from 'package:parallel':

    clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
    clusterExport, clusterMap, parApply, parCapply, parLapply,
    parLapplyLB, parRapply, parSapply, parSapplyLB

The following objects are masked from 'package:stats':

    IQR, mad, xtabs

The following objects are masked from 'package:base':

    Filter, Find, Map, Position, Reduce, anyDuplicated, append,
    as.data.frame, cbind, colnames, do.call, duplicated, eval, evalq,
    get, grep, grepl, intersect, is.unsorted, lapply, lengths, mapply,
    match, mget, order, paste, pmax, pmax.int, pmin, pmin.int, rank,
    rbind, rownames, sapply, setdiff, sort, table, tapply, union,
    unique, unsplit

Loading required package: RSQLite
Loading required package: DBI
Loading required package: ggplot2
Loading required package: reshape2
Loading required package: fastcluster

Attaching package: 'fastcluster'

The following object is masked from 'package:stats':

    hclust

Loading required package: rtracklayer
Loading required package: GenomicRanges
Loading required package: S4Vectors
Loading required package: stats4

Attaching package: 'S4Vectors'

The following objects are masked from 'package:base':

    colMeans, colSums, expand.grid, rowMeans, rowSums

Loading required package: IRanges
Loading required package: GenomeInfoDb
Loading required package: Gviz
Loading required package: grid

Attaching package: 'cummeRbund'

The following object is masked from 'package:GenomicRanges':

    promoters

The following object is masked from 'package:IRanges':

    promoters

The following object is masked from 'package:BiocGenerics':

    conditions

> png(filename="/home/ddbj/snapshot/RGM3/R_BC/result/cummeRbund/csDensity.Rd_%03d_medium.png", width=480, height=480)
> ### Name: csDensity
> ### Title: Density plot of CuffData
> ### Aliases: csDensity csDensity,CuffData-method
> ###   csDensity,CuffFeatureSet-method
> 
> ### ** Examples
> 
> 	a<-readCufflinks(system.file("extdata", package="cummeRbund")) #Create CuffSet object from sample data
> 	genes<-a@genes #Create CuffData object for all 'genes'
> 	d<-csDensity(genes) #Create csDensity plot
> 	d #Render plot
Warning message:
Removed 204 rows containing non-finite values (stat_density). 
> 
> 
> 
> 
> 
> dev.off()
null device 
          1 
>