A class for storing count data obtained from ChIP-seq experiments by
counting the number of reads lying within regions. The class extends
RangedSummarizedExperiment.
Objects from the Class
Objects can be created by calls of the form
ChIPseqSet(chipVals=countDataMatrix,
rowRanges=genomicRegions,
colData=DataFrame(row.names=colnames(countDataMatrix)),
...).
However, one will most likely create a ChIPseqSet object by
calling summarizeReads.
Slots
metadata:
An optional list of arbitrary
content describing the overall experiment.
rowRanges:
Object of class "GRanges" or "GRangesList"
containing the genomic regions where the reads were counted.
colData:
Object of class "DataFrame"
containing information on variable values of the samples. Some methods
require the total library size of each sample stored in a column
titled totalCounts.
assays:
Object of class SimpleList of a
matrix, named chipVals containing the read counts
per genomic region.
Extends
Class "RangedSummarizedExperiment", directly.
Methods
chipVals
signature(object = "ChIPseqSet"): Returns the
matrix with read counts.
chipVals<-
signature(object = "ChIPseqSet", value = "matrix"):
Sets the matrix with read counts.
cpm
signature(object = "ChIPseqSet", libSize,
log2=FALSE, priorCount=0.1): Returns
an object of ChIPseqSet with read counts standardized by
library size - counts per million (cpm). If the library size is
not given, the column sums of the given object are used. Cpm
values are logarithmized after adding priorCounts,
if log2 is TRUE.
Author(s)
Hans-Ulrich Klein (hklein@broadinstitute.org)
See Also
summarizeReads, normalizeChIP
Examples
showClass("ChIPseqSet")
Results
R version 3.3.1 (2016-06-21) -- "Bug in Your Hair"
Copyright (C) 2016 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu (64-bit)
R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.
R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.
Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.
> library(epigenomix)
Loading required package: Biobase
Loading required package: BiocGenerics
Loading required package: parallel
Attaching package: 'BiocGenerics'
The following objects are masked from 'package:parallel':
clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
clusterExport, clusterMap, parApply, parCapply, parLapply,
parLapplyLB, parRapply, parSapply, parSapplyLB
The following objects are masked from 'package:stats':
IQR, mad, xtabs
The following objects are masked from 'package:base':
Filter, Find, Map, Position, Reduce, anyDuplicated, append,
as.data.frame, cbind, colnames, do.call, duplicated, eval, evalq,
get, grep, grepl, intersect, is.unsorted, lapply, lengths, mapply,
match, mget, order, paste, pmax, pmax.int, pmin, pmin.int, rank,
rbind, rownames, sapply, setdiff, sort, table, tapply, union,
unique, unsplit
Welcome to Bioconductor
Vignettes contain introductory material; view with
'browseVignettes()'. To cite Bioconductor, see
'citation("Biobase")', and for packages 'citation("pkgname")'.
Loading required package: S4Vectors
Loading required package: stats4
Attaching package: 'S4Vectors'
The following objects are masked from 'package:base':
colMeans, colSums, expand.grid, rowMeans, rowSums
Loading required package: IRanges
Loading required package: GenomicRanges
Loading required package: GenomeInfoDb
Loading required package: SummarizedExperiment
> png(filename="/home/ddbj/snapshot/RGM3/R_BC/result/epigenomix/ChIPseqSet-class.Rd_%03d_medium.png", width=480, height=480)
> ### Name: ChIPseqSet-class
> ### Title: Class '"ChIPseqSet"'
> ### Aliases: ChIPseqSet-class ChIPseqSet ChIPseqSet,matrix,GRanges-method
> ### ChIPseqSet,matrix,GRangesList-method chipVals<-
> ### chipVals<-,ChIPseqSet,matrix-method chipVals
> ### chipVals,ChIPseqSet-method cpm cpm,ChIPseqSet-method
> ### Keywords: classes
>
> ### ** Examples
>
> showClass("ChIPseqSet")
Class "ChIPseqSet" [package "epigenomix"]
Slots:
Name: rowRanges colData
Class: GenomicRangesORGRangesList DataFrame
Name: assays NAMES
Class: Assays characterORNULL
Name: elementMetadata metadata
Class: DataFrame list
Extends:
Class "RangedSummarizedExperiment", directly
Class "SummarizedExperiment", by class "RangedSummarizedExperiment", distance 2
Class "Vector", by class "RangedSummarizedExperiment", distance 3
Class "Annotated", by class "RangedSummarizedExperiment", distance 4
>
>
>
>
>
> dev.off()
null device
1
>