This class is used in the methylPipe library to point to a
TABIX compressed file containing base-resolution DNA-methylation data
and reference genome sequence
Objects from the Class
Objects can be created by calls of the form new("BSdata", ...)
or using the function BSdata(file, uncov, org) whose arguments are
described in the next section Slots.
Slots
file:
Object of class "character" : the TABIX
compressed file containing base-resolution DNA-methylation data.
This file can be generated through the BSprepare
function, and it must contain the following columns:
chromosome assignment (in the form chr1, chr2...),
genomic position (positive integer),
strand (either - or +),
methylation sequence context (either CG, CHG or CHH),
number (>0) of sequencing reads with C calls at that genomic position,
number of sequencing reads with T calls at that genomic position,
binomial pvalue (-10*log10(pvalue)) for calling a mC at that position.
uncov:
Object of class GRanges : this
GRanges object consists of the list of genomic regions with
sequencing coverage information; this information is used to distinguish
which methylation sites are unmethylated, but covered, from those
that are missing data since they have no sequencing coverage. This
object is automatically generated by the meth.call
function while processing aligned files generated from the aligner.
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> library(methylPipe)
Loading required package: GenomicRanges
Loading required package: BiocGenerics
Loading required package: parallel
Attaching package: 'BiocGenerics'
The following objects are masked from 'package:parallel':
clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
clusterExport, clusterMap, parApply, parCapply, parLapply,
parLapplyLB, parRapply, parSapply, parSapplyLB
The following objects are masked from 'package:stats':
IQR, mad, xtabs
The following objects are masked from 'package:base':
Filter, Find, Map, Position, Reduce, anyDuplicated, append,
as.data.frame, cbind, colnames, do.call, duplicated, eval, evalq,
get, grep, grepl, intersect, is.unsorted, lapply, lengths, mapply,
match, mget, order, paste, pmax, pmax.int, pmin, pmin.int, rank,
rbind, rownames, sapply, setdiff, sort, table, tapply, union,
unique, unsplit
Loading required package: S4Vectors
Loading required package: stats4
Attaching package: 'S4Vectors'
The following objects are masked from 'package:base':
colMeans, colSums, expand.grid, rowMeans, rowSums
Loading required package: IRanges
Loading required package: GenomeInfoDb
Loading required package: SummarizedExperiment
Loading required package: Biobase
Welcome to Bioconductor
Vignettes contain introductory material; view with
'browseVignettes()'. To cite Bioconductor, see
'citation("Biobase")', and for packages 'citation("pkgname")'.
Loading required package: Rsamtools
Loading required package: Biostrings
Loading required package: XVector
> png(filename="/home/ddbj/snapshot/RGM3/R_BC/result/methylPipe/BSdata-class.Rd_%03d_medium.png", width=480, height=480)
> ### Name: BSdata-class
> ### Title: Class "BSdata"
> ### Aliases: BSdata-class BSdata show,BSdata-method
> ### Keywords: classes
>
> ### ** Examples
>
> require(BSgenome.Hsapiens.UCSC.hg18)
Loading required package: BSgenome.Hsapiens.UCSC.hg18
Loading required package: BSgenome
Loading required package: rtracklayer
> H1data <- system.file('extdata', 'H1_chr20_CG_10k_tabix_out.txt.gz', package='methylPipe')
> uncov_GR <- GRanges(Rle('chr20'), IRanges(c(14350,69251,84185), c(18349,73250,88184)))
> H1.db <- BSdata(file=H1data, uncov=uncov_GR, org=Hsapiens)
>
>
>
>
>
> dev.off()
null device
1
>