a list of position frequency matrices, pfms must be a list of class pfm
hc
an object of the type produced by stats::hclust
treewidth
the width to show tree
trueDist
logical flags to use hclust height or not.
...
other parameters can be passed to plotMotifLogo function
Value
none
Examples
#####Input#####
pcms<-readPCM(file.path(find.package("motifStack"), "extdata"),"pcm$")
motifs<-lapply(pcms,pcm2pfm)
#####Clustering#####
jaspar.scores <- MotIV::readDBScores(file.path(find.package("MotIV"),
"extdata", "jaspar2010_PCC_SWU.scores"))
d <- MotIV::motifDistances(lapply(motifs, pfm2pwm))
hc <- MotIV::motifHclust(d, method="average")
##reorder the motifs for plotMotifLogoStack
motifs<-motifs[hc$order]
##do alignment
motifs<-DNAmotifAlignment(motifs)
##plot stacks
plotMotifLogoStack(motifs, ncex=1.0)
plotMotifLogoStackWithTree(motifs, hc=hc)
Results
R version 3.3.1 (2016-06-21) -- "Bug in Your Hair"
Copyright (C) 2016 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu (64-bit)
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Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.
Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.
> library(motifStack)
Loading required package: grImport
Loading required package: grid
Loading required package: XML
Loading required package: MotIV
Loading required package: BiocGenerics
Loading required package: parallel
Attaching package: 'BiocGenerics'
The following objects are masked from 'package:parallel':
clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
clusterExport, clusterMap, parApply, parCapply, parLapply,
parLapplyLB, parRapply, parSapply, parSapplyLB
The following objects are masked from 'package:stats':
IQR, mad, xtabs
The following objects are masked from 'package:base':
Filter, Find, Map, Position, Reduce, anyDuplicated, append,
as.data.frame, cbind, colnames, do.call, duplicated, eval, evalq,
get, grep, grepl, intersect, is.unsorted, lapply, lengths, mapply,
match, mget, order, paste, pmax, pmax.int, pmin, pmin.int, rank,
rbind, rownames, sapply, setdiff, sort, table, tapply, union,
unique, unsplit
Attaching package: 'MotIV'
The following object is masked from 'package:stats':
filter
Loading required package: ade4
Attaching package: 'ade4'
The following object is masked from 'package:BiocGenerics':
score
Loading required package: Biostrings
Loading required package: S4Vectors
Loading required package: stats4
Attaching package: 'S4Vectors'
The following objects are masked from 'package:base':
colMeans, colSums, expand.grid, rowMeans, rowSums
Loading required package: IRanges
Loading required package: XVector
> png(filename="/home/ddbj/snapshot/RGM3/R_BC/result/motifStack/plotMotifLogoStackWithTree.Rd_%03d_medium.png", width=480, height=480)
> ### Name: plotMotifLogoStackWithTree
> ### Title: plot sequence logos stack with hierarchical cluster tree
> ### Aliases: plotMotifLogoStackWithTree
>
> ### ** Examples
>
> #####Input#####
> pcms<-readPCM(file.path(find.package("motifStack"), "extdata"),"pcm$")
> motifs<-lapply(pcms,pcm2pfm)
>
> #####Clustering#####
> jaspar.scores <- MotIV::readDBScores(file.path(find.package("MotIV"),
+ "extdata", "jaspar2010_PCC_SWU.scores"))
> d <- MotIV::motifDistances(lapply(motifs, pfm2pwm))
> hc <- MotIV::motifHclust(d, method="average")
>
> ##reorder the motifs for plotMotifLogoStack
> motifs<-motifs[hc$order]
> ##do alignment
> motifs<-DNAmotifAlignment(motifs)
> ##plot stacks
> plotMotifLogoStack(motifs, ncex=1.0)
> plotMotifLogoStackWithTree(motifs, hc=hc)
>
>
>
>
>
> dev.off()
null device
1
>