Last data update: 2014.03.03

R: plot sequence logos stack with hierarchical cluster tree
plotMotifLogoStackWithTreeR Documentation

plot sequence logos stack with hierarchical cluster tree

Description

plot sequence logos stack with hierarchical cluster tree

Usage

plotMotifLogoStackWithTree(pfms, hc, treewidth=1/8, trueDist=FALSE, ...)

Arguments

pfms

a list of position frequency matrices, pfms must be a list of class pfm

hc

an object of the type produced by stats::hclust

treewidth

the width to show tree

trueDist

logical flags to use hclust height or not.

...

other parameters can be passed to plotMotifLogo function

Value

none

Examples

  #####Input#####
  pcms<-readPCM(file.path(find.package("motifStack"), "extdata"),"pcm$")
  motifs<-lapply(pcms,pcm2pfm)

  #####Clustering#####
  jaspar.scores <- MotIV::readDBScores(file.path(find.package("MotIV"), 
                                 "extdata", "jaspar2010_PCC_SWU.scores"))
  d <- MotIV::motifDistances(lapply(motifs, pfm2pwm))
  hc <- MotIV::motifHclust(d, method="average")

  ##reorder the motifs for plotMotifLogoStack
  motifs<-motifs[hc$order]
  ##do alignment
  motifs<-DNAmotifAlignment(motifs)
  ##plot stacks
  plotMotifLogoStack(motifs, ncex=1.0)
  plotMotifLogoStackWithTree(motifs, hc=hc)

Results


R version 3.3.1 (2016-06-21) -- "Bug in Your Hair"
Copyright (C) 2016 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu (64-bit)

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Type 'demo()' for some demos, 'help()' for on-line help, or
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> library(motifStack)
Loading required package: grImport
Loading required package: grid
Loading required package: XML
Loading required package: MotIV
Loading required package: BiocGenerics
Loading required package: parallel

Attaching package: 'BiocGenerics'

The following objects are masked from 'package:parallel':

    clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
    clusterExport, clusterMap, parApply, parCapply, parLapply,
    parLapplyLB, parRapply, parSapply, parSapplyLB

The following objects are masked from 'package:stats':

    IQR, mad, xtabs

The following objects are masked from 'package:base':

    Filter, Find, Map, Position, Reduce, anyDuplicated, append,
    as.data.frame, cbind, colnames, do.call, duplicated, eval, evalq,
    get, grep, grepl, intersect, is.unsorted, lapply, lengths, mapply,
    match, mget, order, paste, pmax, pmax.int, pmin, pmin.int, rank,
    rbind, rownames, sapply, setdiff, sort, table, tapply, union,
    unique, unsplit


Attaching package: 'MotIV'

The following object is masked from 'package:stats':

    filter

Loading required package: ade4

Attaching package: 'ade4'

The following object is masked from 'package:BiocGenerics':

    score

Loading required package: Biostrings
Loading required package: S4Vectors
Loading required package: stats4

Attaching package: 'S4Vectors'

The following objects are masked from 'package:base':

    colMeans, colSums, expand.grid, rowMeans, rowSums

Loading required package: IRanges
Loading required package: XVector
> png(filename="/home/ddbj/snapshot/RGM3/R_BC/result/motifStack/plotMotifLogoStackWithTree.Rd_%03d_medium.png", width=480, height=480)
> ### Name: plotMotifLogoStackWithTree
> ### Title: plot sequence logos stack with hierarchical cluster tree
> ### Aliases: plotMotifLogoStackWithTree
> 
> ### ** Examples
> 
>   #####Input#####
>   pcms<-readPCM(file.path(find.package("motifStack"), "extdata"),"pcm$")
>   motifs<-lapply(pcms,pcm2pfm)
> 
>   #####Clustering#####
>   jaspar.scores <- MotIV::readDBScores(file.path(find.package("MotIV"), 
+                                  "extdata", "jaspar2010_PCC_SWU.scores"))
>   d <- MotIV::motifDistances(lapply(motifs, pfm2pwm))
>   hc <- MotIV::motifHclust(d, method="average")
> 
>   ##reorder the motifs for plotMotifLogoStack
>   motifs<-motifs[hc$order]
>   ##do alignment
>   motifs<-DNAmotifAlignment(motifs)
>   ##plot stacks
>   plotMotifLogoStack(motifs, ncex=1.0)
>   plotMotifLogoStackWithTree(motifs, hc=hc)
> 
> 
> 
> 
> 
> dev.off()
null device 
          1 
>