The paired-end RNA-seq data is from the RGASP project sample K562_2x75 (replicate 1, lane 1) and was obtained at ftp://ftp.sanger.ac.uk/pub/gencode/rgasp/RGASP1/inputdata/human_fastq. Reads were aligned against hg19 with tophat 2.0.2 and bowtie 0.12.5, setting the insert size at -r 200, and imported into R using scanBam from package Rsamtools. For illustration purposes, we selected reads mapping to a few genes only (namely, the genes that were also selected for the toy genome annotation in data(hg19DB).
We downloaded the fastq files, aligned with TopHat and processed with wrapKnown to obtain the estimated distributions for each of the 6 samples. distrsGSE37704 is a list with the 6 corresponding elements. The estimated distributions for HiSeq data were very similar, hence these distributions can be used as defaults for Illumina MiSeq and HiSeq experiments.
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