Last data update: 2014.03.03

R: qPCR Gene Expression
xqR Documentation

qPCR Gene Expression

Description

An ExpressionSet object containing single-cell gene expression levels measured by qPCR.

Usage

data(xq)

Format

Formal class 'ExpressionSet' [package "Biobase"] with 7 slots
  ..@ experimentData   :Formal class 'MIAME' [package "Biobase"] with 13 slots
  .. .. ..@ name             : chr ""
  .. .. ..@ lab              : chr ""
  .. .. ..@ contact          : chr ""
  .. .. ..@ title            : chr ""
  .. .. ..@ abstract         : chr ""
  .. .. ..@ url              : chr ""
  .. .. ..@ pubMedIds        : chr ""
  .. .. ..@ samples          : list()
  .. .. ..@ hybridizations   : list()
  .. .. ..@ normControls     : list()
  .. .. ..@ preprocessing    : list()
  .. .. ..@ other            : list()
  .. .. ..@ .__classVersion__:Formal class 'Versions' [package "Biobase"] with 1 slots
  .. .. .. .. ..@ .Data:List of 2
  .. .. .. .. .. ..$ : int [1:3] 1 0 0
  .. .. .. .. .. ..$ : int [1:3] 1 1 0
  ..@ assayData        :<environment: 0xb12e938> 
  ..@ phenoData        :Formal class 'AnnotatedDataFrame' [package "Biobase"] with 4 slots
  .. .. ..@ varMetadata      :'data.frame':  4 obs. of  1 variable:
  .. .. .. ..$ labelDescription: chr [1:4] NA NA NA NA
  .. .. ..@ data             :'data.frame':	137 obs. of  4 variables:
  .. .. .. ..$ Sample.ID    : chr [1:137] "34c_2" "34c_3" "34c_4" "34c_5" ...
  .. .. .. ..$ Embryonic.day: chr [1:137] "E3.25" "E3.25" "E3.25" "E3.25" ...
  .. .. .. ..$ Cell.type    : chr [1:137] "ICM" "ICM" "ICM" "ICM" ...
  .. .. .. ..$ sampleGroup  : chr [1:137] "E3.25" "E3.25" "E3.25" "E3.25" ...
  .. .. ..@ dimLabels        : chr [1:2] "sampleNames" "sampleColumns"
  .. .. ..@ .__classVersion__:Formal class 'Versions' [package "Biobase"] with 1 slots
  .. .. .. .. ..@ .Data:List of 1
  .. .. .. .. .. ..$ : int [1:3] 1 1 0
  ..@ featureData      :Formal class 'AnnotatedDataFrame' [package "Biobase"] with 4 slots
  .. .. ..@ varMetadata      :'data.frame':	1 obs. of  1 variable:
  .. .. .. ..$ labelDescription: chr NA
  .. .. ..@ data             :'data.frame':	38 obs. of  1 variable:
  .. .. .. ..$ symbol: chr [1:38] "Fgf4" "Tom1l1" "Tdgf1" "Cldn4" ...
  .. .. ..@ dimLabels        : chr [1:2] "featureNames" "featureColumns"
  .. .. ..@ .__classVersion__:Formal class 'Versions' [package "Biobase"] with 1 slots
  .. .. .. .. ..@ .Data:List of 1
  .. .. .. .. .. ..$ : int [1:3] 1 1 0
  ..@ annotation       : chr "single cell qPCR"
  ..@ protocolData     :Formal class 'AnnotatedDataFrame' [package "Biobase"] with 4 slots
  .. .. ..@ varMetadata      :'data.frame':	0 obs. of  1 variable:
  .. .. .. ..$ labelDescription: chr(0) 
  .. .. ..@ data             :'data.frame':	137 obs. of  0 variables
  .. .. ..@ dimLabels        : chr [1:2] "sampleNames" "sampleColumns"
  .. .. ..@ .__classVersion__:Formal class 'Versions' [package "Biobase"] with 1 slots
  .. .. .. .. ..@ .Data:List of 1
  .. .. .. .. .. ..$ : int [1:3] 1 1 0
  ..@ .__classVersion__:Formal class 'Versions' [package "Biobase"] with 1 slots
  .. .. ..@ .Data:List of 4
  .. .. .. ..$ : int [1:3] 2 15 0
  .. .. .. ..$ : int [1:3] 2 16 0
  .. .. .. ..$ : int [1:3] 1 3 0
  .. .. .. ..$ : int [1:3] 1 0 0

References

Ohnishi et al., 2014

See Also

a, x, xql

Examples

data(xq)
xq
pData(xq)

Results


R version 3.3.1 (2016-06-21) -- "Bug in Your Hair"
Copyright (C) 2016 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu (64-bit)

R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.

R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

> library(Hiiragi2013)
Loading required package: affy
Loading required package: BiocGenerics
Loading required package: parallel

Attaching package: 'BiocGenerics'

The following objects are masked from 'package:parallel':

    clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
    clusterExport, clusterMap, parApply, parCapply, parLapply,
    parLapplyLB, parRapply, parSapply, parSapplyLB

The following objects are masked from 'package:stats':

    IQR, mad, xtabs

The following objects are masked from 'package:base':

    Filter, Find, Map, Position, Reduce, anyDuplicated, append,
    as.data.frame, cbind, colnames, do.call, duplicated, eval, evalq,
    get, grep, grepl, intersect, is.unsorted, lapply, lengths, mapply,
    match, mget, order, paste, pmax, pmax.int, pmin, pmin.int, rank,
    rbind, rownames, sapply, setdiff, sort, table, tapply, union,
    unique, unsplit

Loading required package: Biobase
Welcome to Bioconductor

    Vignettes contain introductory material; view with
    'browseVignettes()'. To cite Bioconductor, see
    'citation("Biobase")', and for packages 'citation("pkgname")'.

Loading required package: boot
Loading required package: clue
Loading required package: cluster
Loading required package: genefilter
Loading required package: geneplotter
Loading required package: lattice

Attaching package: 'lattice'

The following object is masked from 'package:boot':

    melanoma

Loading required package: annotate
Loading required package: AnnotationDbi
Loading required package: stats4
Loading required package: IRanges
Loading required package: S4Vectors

Attaching package: 'S4Vectors'

The following objects are masked from 'package:base':

    colMeans, colSums, expand.grid, rowMeans, rowSums

Loading required package: XML
Loading required package: gplots

Attaching package: 'gplots'

The following object is masked from 'package:IRanges':

    space

The following object is masked from 'package:S4Vectors':

    space

The following object is masked from 'package:stats':

    lowess

Loading required package: gtools

Attaching package: 'gtools'

The following objects are masked from 'package:boot':

    inv.logit, logit

Loading required package: KEGGREST
Loading required package: MASS

Attaching package: 'MASS'

The following object is masked from 'package:AnnotationDbi':

    select

The following object is masked from 'package:genefilter':

    area

Loading required package: mouse4302.db
Loading required package: org.Mm.eg.db


Loading required package: RColorBrewer
Loading required package: xtable
> png(filename="/home/ddbj/snapshot/RGM3/R_BC/result/Hiiragi2013/xq.Rd_%03d_medium.png", width=480, height=480)
> ### Name: xq
> ### Title: qPCR Gene Expression
> ### Aliases: xq
> ### Keywords: datasets
> 
> ### ** Examples
> 
> data(xq)
> xq
ExpressionSet (storageMode: lockedEnvironment)
assayData: 38 features, 137 samples 
  element names: exprs 
protocolData: none
phenoData
  sampleNames: 1 E3.25 2 E3.25 ... 137 E4.5 (EPI) (137 total)
  varLabels: Sample.ID Embryonic.day Cell.type sampleGroup
  varMetadata: labelDescription
featureData
  featureNames: Fgf4 Tom1l1 ... Serpine2 (38 total)
  fvarLabels: symbol
  fvarMetadata: labelDescription
experimentData: use 'experimentData(object)'
Annotation: single cell qPCR 
> pData(xq)
               Sample.ID Embryonic.day Cell.type sampleGroup
1 E3.25            34c_2         E3.25       ICM       E3.25
2 E3.25            34c_3         E3.25       ICM       E3.25
3 E3.25            34c_4         E3.25       ICM       E3.25
4 E3.25            34c_5         E3.25       ICM       E3.25
5 E3.25            34c_6         E3.25       ICM       E3.25
6 E3.25            34c_7         E3.25       ICM       E3.25
7 E3.25            34c_8         E3.25       ICM       E3.25
8 E3.25            41c_1         E3.25       ICM       E3.25
9 E3.25            41c_3         E3.25       ICM       E3.25
10 E3.25           41c_4         E3.25       ICM       E3.25
11 E3.25           41c_5         E3.25       ICM       E3.25
12 E3.25           41c_6         E3.25       ICM       E3.25
13 E3.25           41c_7         E3.25       ICM       E3.25
14 E3.25           41c_8         E3.25       ICM       E3.25
15 E3.25           49c_1         E3.25       ICM       E3.25
16 E3.25           49c_2         E3.25       ICM       E3.25
17 E3.25           49c_3         E3.25       ICM       E3.25
18 E3.25           49c_4         E3.25       ICM       E3.25
19 E3.25           49c_5         E3.25       ICM       E3.25
20 E3.25           49c_6         E3.25       ICM       E3.25
21 E3.25           49c_7         E3.25       ICM       E3.25
22 E3.25           49c_8         E3.25       ICM       E3.25
23 E3.25           49c_9         E3.25       ICM       E3.25
24 E3.25          50c_13         E3.25       ICM       E3.25
25 E3.25          50c_14         E3.25       ICM       E3.25
26 E3.25          50c_15         E3.25       ICM       E3.25
27 E3.25          50c_16         E3.25       ICM       E3.25
28 E3.25          50c_17         E3.25       ICM       E3.25
29 E3.25          50c_18         E3.25       ICM       E3.25
30 E3.25          50c_19         E3.25       ICM       E3.25
31 E3.25          50c_20         E3.25       ICM       E3.25
32 E3.25          50c_21         E3.25       ICM       E3.25
33 E3.25          50c_22         E3.25       ICM       E3.25
34 E3.5 (EPI)      62c_2          E3.5       EPI  E3.5 (EPI)
35 E3.5 (EPI)      62c_3          E3.5       EPI  E3.5 (EPI)
36 E3.5 (PE)       62c_4          E3.5        PE   E3.5 (PE)
37 E3.5 (PE)       62c_5          E3.5        PE   E3.5 (PE)
38 E3.5 (PE)       62c_6          E3.5        PE   E3.5 (PE)
39 E3.5 (EPI)      62c_9          E3.5       EPI  E3.5 (EPI)
40 E3.5 (PE)      62c_10          E3.5        PE   E3.5 (PE)
41 E3.5 (EPI)     62c_11          E3.5       EPI  E3.5 (EPI)
42 E3.5 (PE)      62c_13          E3.5        PE   E3.5 (PE)
43 E3.5 (EPI)     62c_14          E3.5       EPI  E3.5 (EPI)
44 E3.5 (PE)       75c_1          E3.5        PE   E3.5 (PE)
45 E3.5 (PE)       75c_2          E3.5        PE   E3.5 (PE)
46 E3.5 (PE)       75c_3          E3.5        PE   E3.5 (PE)
47 E3.5 (EPI)      75c_4          E3.5       EPI  E3.5 (EPI)
48 E3.5 (EPI)      75c_5          E3.5       EPI  E3.5 (EPI)
49 E3.5 (PE)       75c_6          E3.5        PE   E3.5 (PE)
50 E3.5 (EPI)      75c_8          E3.5       EPI  E3.5 (EPI)
51 E3.5 (EPI)      75c_9          E3.5       EPI  E3.5 (EPI)
52 E3.5 (EPI)     75c_10          E3.5       EPI  E3.5 (EPI)
53 E3.5 (EPI)     75c_11          E3.5       EPI  E3.5 (EPI)
54 E3.5 (EPI)     75c_12          E3.5       EPI  E3.5 (EPI)
55 E3.5 (PE)      75c_13          E3.5        PE   E3.5 (PE)
56 E3.5 (EPI)     75c_14          E3.5       EPI  E3.5 (EPI)
57 E3.5 (EPI)     75c_15          E3.5       EPI  E3.5 (EPI)
58 E3.5 (PE)      75c_16          E3.5        PE   E3.5 (PE)
59 E3.5 (EPI)     75c_17          E3.5       EPI  E3.5 (EPI)
60 E3.5 (EPI)     75c_18          E3.5       EPI  E3.5 (EPI)
61 E3.5 (PE)      75c_19          E3.5        PE   E3.5 (PE)
62 E3.5 (PE)      75c_20          E3.5        PE   E3.5 (PE)
63 E3.5 (PE)       91c_1          E3.5        PE   E3.5 (PE)
64 E3.5 (PE)       91c_2          E3.5        PE   E3.5 (PE)
65 E3.5 (PE)       91c_3          E3.5        PE   E3.5 (PE)
66 E3.5 (PE)       91c_4          E3.5        PE   E3.5 (PE)
67 E3.5 (EPI)      91c_5          E3.5       EPI  E3.5 (EPI)
68 E3.5 (PE)       91c_6          E3.5        PE   E3.5 (PE)
69 E3.5 (EPI)      91c_8          E3.5       EPI  E3.5 (EPI)
70 E3.5 (PE)       91c_9          E3.5        PE   E3.5 (PE)
71 E3.5 (EPI)     91c_10          E3.5       EPI  E3.5 (EPI)
72 E3.5 (PE)      91c_12          E3.5        PE   E3.5 (PE)
73 E3.5 (EPI)     91c_13          E3.5       EPI  E3.5 (EPI)
74 E3.5 (EPI)     91c_14          E3.5       EPI  E3.5 (EPI)
75 E3.5 (PE)      91c_15          E3.5        PE   E3.5 (PE)
76 E3.5 (PE)      91c_16          E3.5        PE   E3.5 (PE)
77 E4.5 (EPI)     163c_1          E4.5       EPI  E4.5 (EPI)
78 E4.5 (EPI)     163c_2          E4.5       EPI  E4.5 (EPI)
79 E4.5 (EPI)     163c_3          E4.5       EPI  E4.5 (EPI)
80 E4.5 (EPI)     163c_4          E4.5       EPI  E4.5 (EPI)
81 E4.5 (EPI)     163c_5          E4.5       EPI  E4.5 (EPI)
82 E4.5 (EPI)     163c_6          E4.5       EPI  E4.5 (EPI)
83 E4.5 (PE)      163c_7          E4.5        PE   E4.5 (PE)
84 E4.5 (PE)      163c_8          E4.5        PE   E4.5 (PE)
85 E4.5 (PE)      163c_9          E4.5        PE   E4.5 (PE)
86 E4.5 (PE)     163c_10          E4.5        PE   E4.5 (PE)
87 E4.5 (PE)     163c_11          E4.5        PE   E4.5 (PE)
88 E4.5 (PE)     163c_12          E4.5        PE   E4.5 (PE)
89 E4.5 (EPI)    163c_13          E4.5       EPI  E4.5 (EPI)
90 E4.5 (PE)     163c_15          E4.5        PE   E4.5 (PE)
91 E4.5 (PE)     163c_16          E4.5        PE   E4.5 (PE)
92 E4.5 (PE)     163c_17          E4.5        PE   E4.5 (PE)
93 E4.5 (PE)     163c_18          E4.5        PE   E4.5 (PE)
94 E4.5 (PE)     163c_19          E4.5        PE   E4.5 (PE)
95 E4.5 (PE)     163c_20          E4.5        PE   E4.5 (PE)
96 E4.5 (PE)     163c_21          E4.5        PE   E4.5 (PE)
97 E4.5 (PE)      207c_1          E4.5        PE   E4.5 (PE)
98 E4.5 (EPI)     207c_2          E4.5       EPI  E4.5 (EPI)
99 E4.5 (PE)      207c_3          E4.5        PE   E4.5 (PE)
100 E4.5 (PE)     207c_4          E4.5        PE   E4.5 (PE)
101 E4.5 (EPI)    207c_5          E4.5       EPI  E4.5 (EPI)
102 E4.5 (EPI)    207c_6          E4.5       EPI  E4.5 (EPI)
103 E4.5 (PE)     207c_7          E4.5        PE   E4.5 (PE)
104 E4.5 (PE)     207c_8          E4.5        PE   E4.5 (PE)
105 E4.5 (EPI)   207c_10          E4.5       EPI  E4.5 (EPI)
106 E4.5 (EPI)   207c_11          E4.5       EPI  E4.5 (EPI)
107 E4.5 (EPI)   207c_12          E4.5       EPI  E4.5 (EPI)
108 E4.5 (EPI)   207c_13          E4.5       EPI  E4.5 (EPI)
109 E4.5 (EPI)   207c_14          E4.5       EPI  E4.5 (EPI)
110 E4.5 (PE)    207c_15          E4.5        PE   E4.5 (PE)
111 E4.5 (PE)    207c_16          E4.5        PE   E4.5 (PE)
112 E4.5 (PE)    207c_17          E4.5        PE   E4.5 (PE)
113 E4.5 (PE)    207c_18          E4.5        PE   E4.5 (PE)
114 E4.5 (PE)    207c_19          E4.5        PE   E4.5 (PE)
115 E4.5 (PE)    207c_20          E4.5        PE   E4.5 (PE)
116 E4.5 (PE)    207c_21          E4.5        PE   E4.5 (PE)
117 E4.5 (PE)    207c_22          E4.5        PE   E4.5 (PE)
118 E4.5 (PE)     227c_2          E4.5        PE   E4.5 (PE)
119 E4.5 (PE)     227c_3          E4.5        PE   E4.5 (PE)
120 E4.5 (PE)     227c_4          E4.5        PE   E4.5 (PE)
121 E4.5 (PE)     227c_5          E4.5        PE   E4.5 (PE)
122 E4.5 (EPI)    227c_6          E4.5       EPI  E4.5 (EPI)
123 E4.5 (EPI)    227c_7          E4.5       EPI  E4.5 (EPI)
124 E4.5 (EPI)    227c_8          E4.5       EPI  E4.5 (EPI)
125 E4.5 (EPI)    227c_9          E4.5       EPI  E4.5 (EPI)
126 E4.5 (EPI)   227c_10          E4.5       EPI  E4.5 (EPI)
127 E4.5 (EPI)   227c_11          E4.5       EPI  E4.5 (EPI)
128 E4.5 (EPI)   227c_12          E4.5       EPI  E4.5 (EPI)
129 E4.5 (EPI)   227c_13          E4.5       EPI  E4.5 (EPI)
130 E4.5 (EPI)   227c_14          E4.5       EPI  E4.5 (EPI)
131 E4.5 (EPI)   227c_15          E4.5       EPI  E4.5 (EPI)
132 E4.5 (EPI)   227c_16          E4.5       EPI  E4.5 (EPI)
133 E4.5 (EPI)   227c_17          E4.5       EPI  E4.5 (EPI)
134 E4.5 (EPI)   227c_18          E4.5       EPI  E4.5 (EPI)
135 E4.5 (PE)    227c_19          E4.5        PE   E4.5 (PE)
136 E4.5 (EPI)   227c_20          E4.5       EPI  E4.5 (EPI)
137 E4.5 (EPI)   227c_21          E4.5       EPI  E4.5 (EPI)
> 
> 
> 
> 
> 
> dev.off()
null device 
          1 
>