R: Bimodal melting curve experiment on the surface of...
DMP
R Documentation
Bimodal melting curve experiment on the surface of microbeads.
Description
A melting curve experiment with six microbead populations and short
oligonucleotide probes (direct hybridization). Detection probes for human
VIM (vimentin), MLC-2v (myosin regulatory light chain 2, ventricular/cardiac
muscle isoform), SERCA2 (Atp2a2 - ATPase, Calcium-transporting ATPase
sarcoplasmic reticulum type, slow twitch skeletal muscle isoform), HRPT1
(hyperparathyroidism 1) and the artificial sequences Poly(dA)20 (20 nt of
dA) and aCS (artificial Control Sequence).
Format
A data frame with the melting curves of six different capture and
detection probe pairs on six microbeads populations for VIM, MLC-2v, SERCA2,
Poly(dA)20, aCS and HPRT1. First column contains the temperature (in degree
Celsius, 1 degree Celsius per step) followed by melting curves of VIM,
MLC-2v, SERCA2, Poly(dA)20, aCS and HPRT1 with bimodal melting pattern. The
dyes and quencher used were Atto 647N and BHQ2.
T
a numeric vector, Temperature in degree Celsius.
VIM.no.Q
a numeric vector, VIM without quencher and without
Poly(dT)20 region.
MLC2v.w.Q.w.dT20
a numeric vector, MLC-2v with
quencher-labeled detection probe and fluorescent Poly(dA)20 detection
probe.
SERCA2.no.Q.w.dT20
a numeric vector, SERCA2 without
quencher-labeled detection probe and Poly(dA)20 detection probe.
PolydA20.w.Q
a numeric vector, Poly(dT)20 with fluorescent
Poly(dA)20 detection probe (quencher labeled).
aCS.w.Q.w.dT20
a numeric vector, artificial Control Sequence
without quencher-labeled detection probe and fluorescent Poly(dA)20
detection probe.
HPRT1.no.Q.w.dT20
a numeric vector, HPRT1 without
quencher-labeled detection probe and fluorescent Poly(dA)20 detection
probe.
Source
Data were measured with the VideoScan platform:
A Highly Versatile Microscope Imaging Technology Platform for the Multiplex
Real-Time Detection of Biomolecules and Autoimmune Antibodies. S. Roediger,
P. Schierack, A. Boehm, J. Nitschke, I. Berger, U. Froemmel, C. Schmidt, M.
Ruhland, I. Schimke, D. Roggenbuck, W. Lehmann and C. Schroeder.
Advances in Biochemical Bioengineering/Biotechnology. 133:35–74,
2013. http://www.ncbi.nlm.nih.gov/pubmed/22437246
Surface Melting Curve Analysis with R. S. Roediger, A. Boehm and I.
Schimke. The R Journal. 5(2):37–52, 2013.
http://journal.r-project.org/
R version 3.3.1 (2016-06-21) -- "Bug in Your Hair"
Copyright (C) 2016 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu (64-bit)
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> library(MBmca)
Loading required package: robustbase
Loading required package: chipPCR
> png(filename="/home/ddbj/snapshot/RGM3/R_CC/result/MBmca/DMP.Rd_%03d_medium.png", width=480, height=480)
> ### Name: DMP
> ### Title: Bimodal melting curve experiment on the surface of microbeads.
> ### Aliases: DMP
> ### Keywords: datasets
>
> ### ** Examples
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> data(DMP)
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> dev.off()
null device
1
>