Last data update: 2014.03.03

R: amplification efficiencies and experimental Cq1 (optional...
amp.effR Documentation

amplification efficiencies and experimental Cq1 (optional column)

Description

Fold-amplification within each PCR cycle for each qRT-PCR assay. Determined by qPCR of serial dilutions of the template (see Pfaffl 2001, Nucleic Acids Res 29:00)

Usage

data(amp.eff)

Format

A data frame with 17 observations on the following 3 variables.

gene

a factor with levels actin adk c3 chrom clect eif3h g3pdh gsp2 hsp16 hsp60 hsp90 nd5 r18s rpl11 spon2 tgoln ubl3

efficiency

a numeric vector

Cq1

a numeric vector

Examples

data(amp.eff)

Results


R version 3.3.1 (2016-06-21) -- "Bug in Your Hair"
Copyright (C) 2016 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu (64-bit)

R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.

R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

> library(MCMC.qpcr)
Loading required package: MCMCglmm
Loading required package: Matrix
Loading required package: coda
Loading required package: ape
Loading required package: ggplot2
> png(filename="/home/ddbj/snapshot/RGM3/R_CC/result/MCMC.qpcr/amp.eff.Rd_%03d_medium.png", width=480, height=480)
> ### Name: amp.eff
> ### Title: amplification efficiencies and experimental Cq1 (optional
> ###   column)
> ### Aliases: amp.eff
> ### Keywords: datasets
> 
> ### ** Examples
> 
> data(amp.eff)
> 
> 
> 
> 
> 
> dev.off()
null device 
          1 
>