R: Murine Secondary Palate Development miRNA Expression Data
PalateData
R Documentation
Murine Secondary Palate Development miRNA Expression Data
Description
This data set contains two-color miRNA microarray expression data
obtained from mouse embryonic tissue during gestational days (GD) 12,
13, and 14, which represents the critical period of palate
development in the mouse.
Usage
data(PalateData)
Format
The data are in the format of an "RGList",
which in this case is a list with the following 9 elements:
R
matrix of dimension 6336 x 9 which contains the red
channel foreground measurements
G
matrix of dimension 6336 x 9 which contains the green
channel foreground measurements
Rb
matrix of dimension 6336 x 9 which contains the red
channel background measurements
Gb
matrix of dimension 6336 x 9 which contains the green
channel background measurements
source
source of the images, here "imagene"
Field.Dimensions
numeric vector giving the field
dimensions of the array (Metarows, Metacols, Rows and Cols)
weights
matrix of dimension 6336 x 9 which contains
the quality weights associated with each spot on the arrays
printer
list containing information on the process
used to print the spots on the arrays (number of grid rows /
columns and number of spot rows / columns per grid - coincides
with Field.Dimensions)
genes
A data.frame containing information on
each probe. Has the following columns:
Field
field position for the probe
Meta Row
meta row position for the probe
Meta Column
meta column position for the probe
Row
row position for the probe
Column
column position for the probe
Gene ID
unique gene identifier provided by Miltenyi Biotec
ID
unique probe identifier constructed by
concatentating the "Gene ID" with "Meta Row", "Meta Column",
"Row", and "Column" information
Name
name of the microRNA
Name.stem
base name of the microRNA
probe.type
type of probe, "MMU miRNAs", "Other
miRNAs", "Control", "Empty", and "Other"
Details
RNA samples were isolated from mouse embryonic orofacial
tissues (GD-12 - GD-14) and fluorescently labeled with Hy5 (red).
Control samples (miRXplore Universal Reference) were labeled with
Hy3 (green). The two sets of samples were hybridized to miRXplore
Microarrays (Miltenyi Biotec) sing the a-Hyb Hybridization Station
(Miltenyi Biotec). Probes for a
total of 1336 mature miRNAs (from human, mouse, rat and virus),
including positive control and calibration probes, were spotted in
quadruplicate on each microarray. Each array included probes for 588
murine miRNAs.
Source
P. Mukhopadhyay, G. Brock, V. Pihur, C. Webb, M.M. Pisano, and R.M. Greene.
Developmental microRNA expression profiling of murine embryonic orofacial tissue.
Birth Defects Res A Clin Mol Teratol, 88(7):511-34, 2010.
Examples
data(PalateData)
Results
R version 3.3.1 (2016-06-21) -- "Bug in Your Hair"
Copyright (C) 2016 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu (64-bit)
R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.
R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.
Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.
> library(MmPalateMiRNA)
Loading required package: Biobase
Loading required package: BiocGenerics
Loading required package: parallel
Attaching package: 'BiocGenerics'
The following objects are masked from 'package:parallel':
clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
clusterExport, clusterMap, parApply, parCapply, parLapply,
parLapplyLB, parRapply, parSapply, parSapplyLB
The following objects are masked from 'package:stats':
IQR, mad, xtabs
The following objects are masked from 'package:base':
Filter, Find, Map, Position, Reduce, anyDuplicated, append,
as.data.frame, cbind, colnames, do.call, duplicated, eval, evalq,
get, grep, grepl, intersect, is.unsorted, lapply, lengths, mapply,
match, mget, order, paste, pmax, pmax.int, pmin, pmin.int, rank,
rbind, rownames, sapply, setdiff, sort, table, tapply, union,
unique, unsplit
Welcome to Bioconductor
Vignettes contain introductory material; view with
'browseVignettes()'. To cite Bioconductor, see
'citation("Biobase")', and for packages 'citation("pkgname")'.
Loading required package: xtable
Loading required package: limma
Attaching package: 'limma'
The following object is masked from 'package:BiocGenerics':
plotMA
Loading required package: statmod
Loading required package: lattice
Loading required package: vsn
> png(filename="/home/ddbj/snapshot/RGM3/R_BC/result/MmPalateMiRNA/PalateData.Rd_%03d_medium.png", width=480, height=480)
> ### Name: PalateData
> ### Title: Murine Secondary Palate Development miRNA Expression Data
> ### Aliases: PalateData
> ### Keywords: datasets
>
> ### ** Examples
>
> data(PalateData)
>
>
>
>
>
> dev.off()
null device
1
>