Melting Curves (resolution: 0.1 degrees Celsius / step) for the Gene adk Using
the Bio-Rad CFX96 thermo cycler
Description
A melting curve for adk was performed.
Usage
data("C317.melt.hr")
Format
A data frame with 40 observations on the following 97 variables. The first
column ("Temperature") contains the temperature (resolution: 0.1 degrees Celsius / step)
and consecutive columns
contain the replicates ("A1" to "H12").
Details
adk was amplified in the Bio-Rad CFX96. After PCR, the temperature-dependent
change of fluorescence was simultaneously monitored (EvaGreen, Mao et al. 2007).
The primer sequences for adk were taken from this study.
Stefan Roediger, Claudia Deutschmann, Claudia Zelck (BTU Cottbus - Senftenberg)
References
A Highly Versatile Microscope Imaging Technology Platform for the Multiplex
Real-Time Detection of Biomolecules and Autoimmune Antibodies. S. Roediger,
P. Schierack, A. Boehm, J. Nitschke, I. Berger, U. Froemmel, C. Schmidt,
M. Ruhland, I. Schimke, D. Roggenbuck, W. Lehmann and C. Schroeder.
Advances in Biochemical Bioengineering/Biotechnology. 133:33–74, 2013.
http://www.ncbi.nlm.nih.gov/pubmed/22437246
Mao, F., Leung, W.-Y., Xin, X., 2007. Characterization of EvaGreen and the
implication of its physicochemical properties for qPCR applications.
BMC Biotechnol. 7, 76.