R: Recombinase polymerase amplification (RPA) by Lutz et...
RPA
R Documentation
Recombinase polymerase amplification (RPA) by Lutz et al.(2009)
Description
Real-time amplification plot of an Recombinase Polymerase Amplification (RPA)
by Lutz et al.(2009) in a centrifugal microfluidic foil cartridge.
Usage
data("RPA")
Format
A data frame with 184 observations on the following 2 variables.
Reaction.Time
Reaction Time [min]
RFU
Relative fluorescence units [RFU]
Details
The data were digitized as described by Poisot (2011). The image for data
extraction was taken from Figure 3b by Lutz et al.(2009). The amplification
curve present the results of a cross-contamination test of a foil disk for a
sample containing 2000 copies of the mecA gene. For further experimental and
technical details refer to Lutz et al.(2009).
Source
The original data (open access under CC BY-NC-ND license) were taken from Figure
3b by Lutz et al.(2009).
References
Poisot, T. (2011). The digitize Package: Extracting Numerical Data from
Scatterplots. The R Journal 3, 25–26.
Lutz, S., Weber, P., Focke, M., Faltin, B., Roth, G., Piepenburg, O., Armes,
N., Mark, D., Zengerle, R., and von Stetten, F. (2009). Isothermal Polymerase
Amplification in a Centrifugal Microfluidic Foil Cartridge. Procedia
Chemistry 1, 529–531.
Examples
data(RPA)
plot(RPA, main = "RPA by Lutz et al.(2009)", xlab = "Reaction Time [min]",
ylab = "Relative fluorescence units [RFU]", type = "l")
abline(h = 5, lty = 2, col = "grey")