Data set of an amplification reaction using the Bio-Rad CFX96 thermo cycler.
The samples of Vimentin were amplified in the CFX96 as replicates according to
Roediger et al. (2013). The quantification was performed during the annealing
step (60 degrees Celsius).
Usage
data(VIMCFX96_60)
Format
A data frame with 40 observations on the following 97 variables. The first
column ("Cycle") contains the number of cycles and consecutive columns
contain the replicates ("A1" to "H12").
Source
Stefan Roediger, Claudia Deutschmann (BTU Cottbus - Senftenberg)
References
A Highly Versatile Microscope Imaging Technology Platform for the Multiplex
Real-Time Detection of Biomolecules and Autoimmune Antibodies. S. Roediger,
P. Schierack, A. Boehm, J. Nitschke, I. Berger, U. Froemmel, C. Schmidt,
M. Ruhland, I. Schimke, D. Roggenbuck, W. Lehmann and C. Schroeder.
Advances in Biochemical Bioengineering/Biotechnology. 133:33–74, 2013.
http://www.ncbi.nlm.nih.gov/pubmed/22437246
Examples
data(VIMCFX96_60)
data(VIMCFX96_69)
T60 <- rowMeans(VIMCFX96_60[, 2:ncol(VIMCFX96_60)])
T69 <- rowMeans(VIMCFX96_69[, 2:ncol(VIMCFX96_69)])
plot(1:length(T60), T60, main = "Fluorescence at different
temperatures\nQuantification in CFX96 (Bio-Rad)", xlab = "Cycle",
ylab = "Cycle dependent fluorescence", pch = 15, type = "b")
lines(1:length(T69), T69, pch = 19, type = "b", col = 2)
legend(1, 4500, c("Annealing (60 deg C)", "Elongation (69 deg C)"),
pch = c(15, 19), col = c(1,2))