Data set of an amplification reaction using the Bio-Rad iQ5 thermo cycler. The
samples of Vimentin were amplified in the iQ5 as replicates according to
Roediger et al. (2013). The quantification was performed during the annealing
step (59.5 degrees Celsius).
Usage
data(VIMiQ5_595)
Format
A data frame with 40 observations on the following 97 variables. The first
column ("Cycles") contains the number of cycles and consecutive columns
contain the replicates ("A01" to "H12").
Source
Stefan Roediger, Claudia Deutschmann (BTU Cottbus - Senftenberg)
References
A Highly Versatile Microscope Imaging Technology Platform for the Multiplex
Real-Time Detection of Biomolecules and Autoimmune Antibodies. S. Roediger,
P. Schierack, A. Boehm, J. Nitschke, I. Berger, U. Froemmel, C. Schmidt,
M. Ruhland, I. Schimke, D. Roggenbuck, W. Lehmann and C. Schroeder.
Advances in Biochemical Bioengineering/Biotechnology. 133:33–74, 2013.
http://www.ncbi.nlm.nih.gov/pubmed/22437246
Examples
T595 <- rowMeans(VIMiQ5_595[, 2:ncol(VIMiQ5_595)])
T685 <- rowMeans(VIMiQ5_685[, 2:ncol(VIMiQ5_685)])
plot(1:length(T595), T595, main = "Fluorescence at different
temperatures\nQuantification in iQ5 (Bio-Rad)", xlab = "Cycle",
ylab = "Cycle dependent fluorescence", pch = 15, type = "b")
lines(1:length(T685), T685, pch = 19, type = "b", col = 2)
legend(1, 10000, c("Annealing (59.5 deg C)", "Elongation (68.5 deg C)"),
pch = c(15, 19), col = c(1,2))